椰汁对大花蕙兰超含水态苗发生影响研究  

Effects of Coconut Milk on Plantlet Hyperhydricity of Cymbidium Hybrid

作　　者：杨传贵[1,2] 刘景然[3] 刘雪梅[2] 高玉葆[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]天津城市建设学院规划与建筑系,天津300384 [3]天津蓟县林业局,天津蓟县301909

出　　处：《南开大学学报（自然科学版）》2011年第3期85-89,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市科技攻关项目(07YFGZNC00900)

摘　　要：研究了椰汁对4个大花蕙兰杂交系无菌播种超含水态苗发生的影响,发现椰汁是引起大花蕙兰小植株超含水态发生的重要因素.椰汁用量不同,不同杂交组合超含水态发生情况差异较大,最高发生率达到37.0%,最低为零.椰汁对超含水态的发生具有重要影响.当激素6-BA和IBA用量一定时,在3.0%～6.0%范围内,随着椰汁用量的增加,超含水态苗数量升高,超含水态发生指数最高达到26.0.分析表明,椰汁中含有的激素、糖类和氨基酸,引起培养基中激素、糖类和铵离子浓度的增加,进而引起超含水态现象.Chose 4 hybrid groups of Cymbidium,and explored the effects of coconut milk on hyperhydricity of Cymbidium plantlet in vitro.Reveals firstly that coconut milk is an important factor causing hyperhydricity of Cymbidium plantlet.It showed that different hybrid groups differed in hyperhydricity occurrence with the content variation of coconut milk.The highest rate reached 37.0%,while the lowest one was zero.Coconut milk has great effects on the occurrence of hyperhydricity.When the quantity of 6-BA and IBA were fixed,the number of plantlets infected by hyperhydricity increased with the increasing of coconut milk within 3.0%～6.0%,in which the highest hyperhydricity index got 26.0.We realize that the hormone, sugars,and amino acids contained in coconut milk raised the level of hormone,sugars, and NH concentrations in the culture media,and resulted high hyperhydricity occurrence.The paper created the idea of hyperhydricity index which evaluates the occurrence of hyperhydricity, and get accurate and better appraisement.

关 键 词：大花蕙兰 超含水态 椰汁 继代 

分 类 号：Q945[生物学—植物学]