血吸虫合成多肽抗原保护性免疫效果及小鼠外周血细胞的变化(英文)  

THE EFFECT OF PROTECTIVE IMMUNITY OF MULTIPLE ANTIGEN PEPTIDE OF SCHISTOSOMA AND THE CHANGE OF BLOOD CELLS IN MICE

作　　者：苏崇杰 江月 熊国干 石佑恩[2] 宁长修[2] 何昌浩[2] 曹胜利[3] 蔡孟深[3] 秦致辉[3] 

机构地区：[1]武汉市职工医学院生物教研室,武汉430016 [2]同济医科大学寄生虫学教研室 [3]北京医科大学药学院生物有机化学系

出　　处：《中国寄生虫病防治杂志》1999年第4期297-299,共3页Chinese Journal of Parasitic Disease Control

摘　　要：根据公认的血吸虫保护性抗原候选分子Ｓｍ２８ 的氨基酸序列人工合成４ 个不同血吸虫多肽片段，对小鼠进行免疫保护实验。采用２００ μｇ／只，皮下注射共免疫３ 次，每次间隔１ 周，末次免疫后２ 周以（４０ ±２） 条日本血吸虫正常尾蚴攻击感染。另设不免疫攻击感染日本血吸虫的小鼠为对照组。实验组小鼠分别获得２４．３ ％ ～４７ ．９ ％ 的减虫率和３５．１ ％ ～５０ ．４ ％ 的减卵率。各组小鼠免疫前和免疫后尾部取血涂片染色，观察比较外周血白细胞及Ｔ 细胞、Ｂ细胞的变化，结果免疫后较免疫前嗜酸性白细胞和Ｔ细胞升高。实验提示，血吸虫多肽抗原有一定免疫效果，值得进一步研究。The experiment of immune protection for mice was done with four types of synthetic multiple antigen peptide. Each mouse was injected subcutaneously with 200 μg for three times with an interval of one week. After two weeks from the last immunization, they were challenged by (40±2) cercariae of Schistosoma japonicum . Meanwhile, unimmunized mice were treated as control. In immunized group, worm reduction rate was 24.3%-47.9%,and egg reduction rate 35.1%-50.4%. Blood was collected from mice in each group before and after the test, and blood smears were stained. The changes of leucocyte, T cells and B cells from peripheral blood were compared, and it was found that eosinophil and T cell increased after the immunization. This experiment has shown that the multiple antigen peptide of Schistosoma is quite good for immunity and worth for further study.

关 键 词：日本血吸虫 多肽抗原 T细胞 保护性免疫 

分 类 号：R532.210.1[医药卫生—内科学]