应用IC-RT-PCR方法检测水仙中百合斑驳病毒  

Detection of Lily Mottle Virus in Narcissus by the Method of IC-RT-PCR

作　　者：贾丽霞[1] 李志勇[2] 马继芳[2] 

机构地区：[1]河北工程大学,河北邯郸056038 [2]河北省农林科学院谷子研究所,河北石家庄050031

出　　处：《北方园艺》2011年第14期123-125,共3页Northern Horticulture

基　　金：国家"863"计划资助项目(2008AA100905-21);河北邯郸市科技局基金资助项目(0923130070-3)

摘　　要：水仙病毒严重影响水仙的品质与规模化生产，而快速检测水仙病毒可以有效防控其危害与扩散。根据已报道的百合斑驳病毒外壳蛋白基因序列设计PCR引物，利用免疫捕获PCR检测了水仙样品中百合斑驳病毒。PCR扩增产物回收后克隆到PMD19-T载体中测序。把克隆得到的百合斑驳病毒外壳蛋白基因部分序列提交到Genbank中。结果表明：基因登录号为JF714974，克隆的百合斑驳病毒外壳蛋白基因部分序列与国内外百合斑驳病毒同源性为90．4%～99．5％。建立了水仙中百合斑驳病毒的IC-RT-PCR的检测方法，简化了试验步骤，适合于水仙中百合斑驳病毒的快速检测。The virus on narcissus affect the quality and growth of narcissus at large scale. The quick detection and identification of narcissus virus is important for control of the disease. According to reported lily mottle virus（LMoV） coat protein sequence,a pairs of primer was desingned and IC-RT-PCR was carried out. The sample collected from Handan were detected with the primer. IC-RT-PCR products were purified and ligated into PMD19-T vector. The partial coat protein gene sequence was submited to Genbank. The results showed that the accession number was JF714974. The gene alignment analysis showed handan isolate shared 90.4%-99. 5 % homology with coat protein gene of other lily mottle virus isolate. The study constructed the platform of detection of lily mottle virus by IC-RT- PCR which simplified procedure and it was convenient for quickly detection of LMoV in narcissus.

关 键 词：水仙病毒病 免疫捕获PCR 百合斑驳病毒 序列分析 

分 类 号：S432.4[农业科学—植物病理学]