碳青霉烯耐药鲍曼不动杆菌耐药性分析及金属β-内酰胺酶检测  

Detection of Metallo-β-lactamases produced by carbapenem-resistant Acinetobacter baumannii

作　　者：汤荣睿[1,2] 龚雅利[1] 张晓兵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]重庆市沙坪坝区人民医院检验科,400030

出　　处：《重庆医学》2011年第21期2094-2095,2098,共3页Chongqing medicine

摘　　要：目的研究碳青霉烯类耐药的鲍曼不动杆菌耐药性及产金属β-内酰胺酶情况。方法采用K-B法(CLSI 2008年标准)筛选对碳青霉烯类耐药的鲍曼不动杆菌临床分离株;用乙二胺四乙酸(EDTA)抑制试验测定鲍曼不动杆菌产金属β-内酰胺酶的情况;PCR方法检测IMP-1、IMP-2、VIM、VIM-2金属酶。结果 51株碳青霉烯类耐药鲍曼不动杆菌为多重耐药菌株,除阿米卡星外,其他抗生素的敏感率都很低;IMP-1、VIM-2基因阳性率分别为25.5%、19.6%;IMP-2、VIM基因均为阴性。结论产生金属β-内酰胺酶是西南医院鲍曼不动杆菌对碳青霉烯类耐药的主要机制之一。碳青霉烯耐药鲍曼不动杆菌是院内感染的多重耐药株,值得关注。Objective To investigate antimicrobial resistance and detection of Metallo-β-lactamases produced by carbapenem-resistant Acinetobacter baumannii.Methods Antimicrobial susceptibility test was done on 51 strains of Acinetobacter baumannii isolated by Kirby-Bauer method.Results were assessed according to the standands recommended by CLSI（2008）.Metallo-β-lactamases was detected by EDTA inhibition test.The genotype of IMP-1,IMP-2,VIM,VIM-2 was performed by polymerase chain reaction（PCR）.Results 51 strains Acinetobacter baumannii were multi-drug resistant.Sensitive to antibiotics except Amikacin were low.Positive rate of strains of IMP-1,VIM-2 was 25.5%,19.6%,respectively.But no IMP-2,VIM was detected.Conclusion Production of Metallo-β-lactamases in Acinetobacter baumannii is one of the main mechanisms of carbapenems resistance at our hospita1.It brings concern that carbapenem-resistant clone was multipleresistant strains.

关 键 词：鲍曼不动杆菌 碳青霉烯类 耐药性 Β-内酰胺酶 

分 类 号：R446.5[医药卫生—诊断学]