南方型紫花苜蓿耐盐细胞系的筛选及生理特性分析  

Selection and Physiological Characterization of Salt-tolerant Cell Line of Southern Type Alfalfa

作　　者：马进[1] 刘志高[1] 郑钢[1] 

机构地区：[1]浙江农林大学园林学院,浙江临安311300

出　　处：《中国草地学报》2011年第4期68-72,共5页Chinese Journal of Grassland

基　　金：浙江省自然科学基金"耐南方滩涂种植的紫花苜蓿细胞突变体筛选及机理研究"(Y304072);"南方型紫花苜蓿耐盐突变体盐胁迫特异蛋白及相关基因筛选与鉴定"(Y3090498);国家"十二五"科技支撑计划项目"中东部地区优质紫花苜蓿高效生产利用关键技术研究与集成示范"(2011BAD17B04)

摘　　要：以NaCl为选择因子,对长期继代培养的紫花苜蓿愈伤组织进行选择,筛选出耐1.2%NaCl的耐盐细胞系并对其进行生理分析,结果表明:耐盐细胞系对逆境的忍受能力高于对照,积累的脯氨酸和可溶性蛋白含量也高于对照,维持着较高的K+/Na+比,在盐分胁迫下能维持较高的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性。Calluses of Southern type alfalfa cultured for a long term were selected,a cell line tolerant to 1.2% NaCl was isolated by using NaCl as selection pressure.The physiological and biochemical properties of this salt tolerant cell line were analyzed.The results showed that the tolerance of the salt-tolerant cell line was much higher than that of control line.Free proline content and soluble protein content increased in salt-tolerant cell line was higher than that in control,a higher K＋/Na＋ ratio was kept in the salt-tolerant cell line.The activities of superoxide dismutase（SOD）,peroxidase（POD） in salt-tolerant cell line were higher than that of the control under NaCl stress.

关 键 词：南方型紫花苜蓿 耐盐细胞系 筛选 生理特性 

分 类 号：S543[农业科学—作物学] Q945.78[生物学—植物学]