Genetic Variation Among the Geographic Population of the Grain Aphid, Sitobion avenae (Hemiptera: Aphididae) in China Inferred from Mitochondrial COI Gene Sequence  

作　　者：XU Zhao-huan CHEN Ju-lian CHENG Deng-fa LIU Yong Frédéric Francis 

机构地区：[1]College of Plant Protection, Shandong Agricultural University, Tai＇an 271018, P.R.China [2]State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China [3]Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, Gembloux B-5030, Belgium

出　　处：《Agricultural Sciences in China》2011年第7期1041-1048,共8页中国农业科学（英文版）

基　　金：supported by the National Basic Research Program of China (973 Program, 2006CB102004);the National S&T Major Project of China (2009ZX08012-007B);the National Natural Science Foundation of China (30971920);the Inter-University Targeted Project between Belgium and China (PIC SHANDONG)

摘　　要：In order to characterize the genetic relationship of the geographic populations of Sitobion avenae （Hemiptera： Aphididae） in China, a 588 bp region of the mitochondrial cytochrome oxidase subunit I （mtDNA-COI） gene was sequenced and analyzed among the different geographic populations. 269 individuals were collected from 17 localities in different wheat-growing areas in China that covered most of the range reported for this species. Within the sequence among these geographic populations, 15 polymorphic sites defined 16 distinct haplotypes, ranging in sequence divergence from 0.2% （one nucleotide） to 1.7% （10 nucleotides）. Of the 15 variable sites, 12 were transitional substitutions, 2 were transversional substitutions and 1 was transitional and transversional substitution. Phylogenetic analysis showed that all haplotypes were highly interconnected with each other, in absence of phylogeographic structing. Each of 8 haplotypes was found only at one locality, and the other haplotypes were the widespread distributed in the different localities. The higher genetic diversity was found in the northern China populations than that in the southern China populations. The low genetic differentiation （FST=-0.06945-0.69857） and high migration rate （Nm=0.21575-infinite） of Chinese populations suggest that dispersal over long distance is a major factor in the demography of S. avenae.In order to characterize the genetic relationship of the geographic populations of Sitobion avenae （Hemiptera： Aphididae） in China, a 588 bp region of the mitochondrial cytochrome oxidase subunit I （mtDNA-COI） gene was sequenced and analyzed among the different geographic populations. 269 individuals were collected from 17 localities in different wheat-growing areas in China that covered most of the range reported for this species. Within the sequence among these geographic populations, 15 polymorphic sites defined 16 distinct haplotypes, ranging in sequence divergence from 0.2% （one nucleotide） to 1.7% （10 nucleotides）. Of the 15 variable sites, 12 were transitional substitutions, 2 were transversional substitutions and 1 was transitional and transversional substitution. Phylogenetic analysis showed that all haplotypes were highly interconnected with each other, in absence of phylogeographic structing. Each of 8 haplotypes was found only at one locality, and the other haplotypes were the widespread distributed in the different localities. The higher genetic diversity was found in the northern China populations than that in the southern China populations. The low genetic differentiation （FST=-0.06945-0.69857） and high migration rate （Nm=0.21575-infinite） of Chinese populations suggest that dispersal over long distance is a major factor in the demography of S. avenae.

关 键 词：Sitobion avenae insect pest mitochondrial DNA mtDNA-COI gene geographic variation 

分 类 号：Q78[生物学—分子生物学] P425.55[天文地球—大气科学及气象学]