Immunoproteomic Assay of Antigenic Surface Proteins in Streptococcus equi ssp. zooepidemicus  

作　　者：MAO Ying FAN Hong-jie ZHOU Yong-hua LU Cheng-ping 

机构地区：[1]Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture/College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China [2]Jiangsu Institute of Parasitic Diseases,Wuxi 214064, P.R.China

出　　处：《Agricultural Sciences in China》2011年第7期1096-1105,共10页中国农业科学（英文版）

基　　金：supported by the Program for New Century Excellent Talents (NCET) at the University of China (NCET-08-0794);the Key Technology R&D Program of Jiansu Province, China (BE2009388);the Fundamental Research Funds for the Central Universities, China (KYT 201003);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China

摘　　要：Streptococcus equi ssp. zooepidemicus （S. zooepidemicus） is a zoonotic pathogen with worldwide distribution. Lacking suitable vaccine and virulent maker is still bottleneck to control this infection. An immunoproteomic approach has been used to screen the membrane-associated and cell wall-associated proteins of S. zooepidemicus isolate in China CY to discover vaccine candidate antigens and therapeutic agents. Finally, 11 membrane-associated proteins, and 13 cell wall-associated proteins were successfully identified. BLAST （www.sanger.ac.uk） results also indicated that nucleotide sequences of majority identified proteins shared high homology （60%） with S. zooepidemicus, except for AC1-3, AC5, AC8, and AC13. Moreover, genes for 7 of the identified proteins were detected from CY; compared with ST171, 3 of them （AM1, AM8 and AC11） were only found in virulent strains （CY）. All of the proteins identified in this study remain not to be reported in S. zooepidemicus. Some of the proteins serve a vital role in the immune system and reproduction of host species according to available data, while the functions of the rest were seldom researched.Streptococcus equi ssp. zooepidemicus （S. zooepidemicus） is a zoonotic pathogen with worldwide distribution. Lacking suitable vaccine and virulent maker is still bottleneck to control this infection. An immunoproteomic approach has been used to screen the membrane-associated and cell wall-associated proteins of S. zooepidemicus isolate in China CY to discover vaccine candidate antigens and therapeutic agents. Finally, 11 membrane-associated proteins, and 13 cell wall-associated proteins were successfully identified. BLAST （www.sanger.ac.uk） results also indicated that nucleotide sequences of majority identified proteins shared high homology （60%） with S. zooepidemicus, except for AC1-3, AC5, AC8, and AC13. Moreover, genes for 7 of the identified proteins were detected from CY; compared with ST171, 3 of them （AM1, AM8 and AC11） were only found in virulent strains （CY）. All of the proteins identified in this study remain not to be reported in S. zooepidemicus. Some of the proteins serve a vital role in the immune system and reproduction of host species according to available data, while the functions of the rest were seldom researched.

关 键 词：membrane-associated proteins （MAP） cell wall-associated proteins （CP） IMMUNOPROTEOMICS S. zooepidemicus 

分 类 号：S852.723[农业科学—基础兽医学] TQ461.1[农业科学—兽医学]