白藜芦醇诱导白血病Molt-4细胞凋亡及对WAVE1基因表达的影响  

Apoptosis of leukemia Molt-4 cells induced by resveratrol and effect of WAVE1 gene expression

作　　者：李永军[1] 姚君霞[1] 

机构地区：[1]河北医科大学第二医院检验科,石家庄050000

出　　处：《白血病．淋巴瘤》2011年第7期389-391,394,共4页Journal of Leukemia & Lymphoma

摘　　要：目的探讨白藜芦醇诱导人类急性淋巴细胞白血病Molt-4细胞凋亡的作用机制。方法噻唑蓝比色法（MTY）测定细胞生长抑制率；流式细胞术检测细胞周期分布及凋亡率；半定量反转录聚合酶链反应（RT—PCR）法检测WAVEl基因的表达。结果MTY结果显示：12．5、25．0、50．0、100．0、200．0μmol／L的白藜芦醇作用于Molt-4细胞24、48、72h后，细胞的最大抑制率分别达到29．32％、36．11％、53．92％、62．50％、74．98％，并呈时间．剂量依赖性（F=33.614，P〈0．05）；流式细胞术检测结果显示：与对照组相比，50．0、100．0μmol／L白藜芦醇作用于Molt-4细胞48h后，S期细胞比例由42．2％分别上升为68．6％和78．1％，细胞发生了S期阻滞（F=19．453，P〈0．01）；PCR结果显示：50．0、100．0μmol／L的白藜芦醇处理Molt-4细胞48h后，WAVEl／GAPDH比值分别为0．356±0．03、0．382±0．05，与对照组（0．586±0．06）比较，差异有统计学意义（F=8．950，P〈0．01）。结论白藜芦醇可诱导Molt-4细胞发生凋亡，其作用机制可能与下调WAVE1基因表达有关。Objective To investigate the possible mechanism of apoptosis effect induced by resveratrol in human acute T lymphoblast leukemia Moh-4 cells. Methods MTT method was used to analyze the inhibition rate. The cell cycle and apoptosis percentage of Molt-4 cells treated with resveratrol were detected by flow eytometry. The expression of WAVE1 mRNA was assessed by semi-quantitative RT-PCR. Results After treated with 12.5, 25.0, 50.0, 100.0, 200.0 μmol/L resveratrol for 24, 48 and 72 hours, the inhibitory effects were at 29.32 %, 36.11 %, 53.92 %, 62.50 %, and 74.98 %, respectively. Resveratrol was able to inhibit the proliferation of Moh-4 cells in a time-dependent and dose-dependent manner （F =33.614, P 〈0.05）. Compared with control group, after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the cell number in S phase of Molt-4 cells was 68.6 % and 78.1%, respectively, and the cell cycle was arrested at S phase （F = 19.453, P 〈 0.01） after treated with 50.0, 100.0 μmol/L resveratrol for 48 h, the ratio of WAVE1/GAPDH was 0.356±0.03, 0.382±0.05. Compared with control group 0.586±0.06, the ratio of WAVE1/GAPDH was decreased （F =8.950, P 〈0.01）. Conclusion Resveratrol could induce the cells to undergo apoptosis, which was probably related to downregulation the expression of WAVE1.

关 键 词：肿瘤 实验性 白藜芦醇 MOLT-4细胞 细胞凋亡 WAVE1基因 

分 类 号：R285[医药卫生—中药学]