中国荷斯坦牛DGAT2基因第4外显子与产奶性状的关联分析  

Genetic Effects of DGAT2 Gene Exon4 on Milk Production Traits in Chinese Holstein Cows

作　　者：赵国丽[1] 李鹏 马秋萌[1] 宫艳斌[1] 韩元[1] 顾亚玲[1] 史远刚[1,2,3] 

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏正鑫牧业科技发展有限公司,银川750002 [3]宁夏四正生物工程技术研究中心,银川750200

出　　处：《中国奶牛》2011年第14期5-7,共3页China Dairy Cattle

基　　金：现代农业(奶牛)产业技术体系建设项目(nycytx-0106);宁夏回族自治区科技攻关计划

摘　　要：本试验以宁夏地区8个公牛家系的各8头女儿共计64头中国荷斯坦母牛为研究对象,利用ABI3730测序仪对DGAT2基因Exon4进行了基因型检测后发现三个突变位点474bp(C/G)、481bp(G/A)、621bp(C/T),各个位点发现有两种基因型,分别为CC型、GC型,GG型、GA型,CC型和CT型,基因型频率分别为0.484、0.516,0.687、0.313,0.484和0.516。通过SAS软件(8.02)对4个产奶性状与DGAT2基因Exon4的关联分析表明,474bp(C/G)CG型305d产奶量和乳脂率显著高于CC型(P<0.05),乳蛋白率、体细胞数影响不显著(P>0.05);481bp(G/A)GA型305d产奶量显著高于GG型,与乳脂率、乳蛋白率、体细胞数无显著的相关性(P>0.05);621bp(C/T)CT型305d产奶量、乳蛋白率、体细胞数都显著高于CC型(P<0.05),乳脂率影响不显著(P>0.05)。With the ABI3730 DNA Squeneer, three mutations of DGAT2 Exon4 had been detected in 64 Chinese Holstein cows from eight sire famailies. The three SNPs including 474bp（C/G） ,481 bp （G/A） and 621bp（C/T） had been genotyped as CC, GC ; GG, GA; CC, CT and their frequency were 0.484,0.516;0.687,0.313;0.484,0.516 respectively; Further, the genetic effects of the DGAT2 Exon4 mutations on four milk production traits were estimated using SAS（8.02）soflware. Statistical results indicated that 305-day milk yield and fat ratio of CG genotype cows were higher than CC genotype（P〈0.05）in 474bp（C/G） mutation; 305-day milk yield of the genotype GA was higher than GG genotype（P〈0.05） in 481bp（G/A） mutation; 305-day milk yield, protein ratio and somatic cell count of the genotype CT were higher than CC genotype（P〈0.05） in 621bp（C/T） mutation.

关 键 词：中国荷斯坦牛 DGAT2基因 产奶性状 关联分析 

分 类 号：S823[农业科学—畜牧学]