传染性法氏囊病毒VP2蛋白的表达及间接ELISA抗体检测方法的建立  

Establishment of an indirect ELISA method using a infectious bursal disease virus VP2 protein as antigen

作　　者：宋军[1] 黄成斌[1] 单雪芹[1] 潘玲[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2011年第4期633-636,共4页Journal of Anhui Agricultural University

摘　　要：建立以传染性法氏囊病毒(IBDV)VP2蛋白为诊断抗原的传染性法氏囊病(IBD)血清学检测方法,研究了VP2蛋白的编码基因,并在原核表达系统中表达了VP2蛋白。用表达的VP2蛋白建立了IBDV间接ELISA检测方法。抗原最佳包被量为每孔174.67 ng,血清最佳稀释度为1:40。对采自安徽部分地区的979鸡血清样品进行检测,IBDV抗体阳性率为39.73%。结果证实,间接ELISA法用于IBD血清抗体的监测具有良好的特异性,是一种快速简便的血清学方法,值得在基层推广应用。The VP2 protein of infectious bursal disease virus（IBDV） is highly conserved and could be used as diagnostic antigen in serological test.In this study,a VP2 protein gene was cloned and expressed in Escherichia coli.The expressed protein was used to establish an indirect ELISA method.The optimal coating concentration of antigen was amounted to 174.67 ng of VP2 protein per well,and the serum sample for testing was diluted to 1：40.A total of 979 serum samples originated from different areas in Anhui province were detected by this indirect ELISA,and the positive rate was 39.73%.The results confirmed that indirect ELISA was specific to monitor se-rum antibody of IBD.This detection method is quick and easy,and it is deserved to be popularized.

关 键 词：传染性法氏囊病病毒 VP2蛋白 间接ELISA 抗体检测 

分 类 号：S852.657[农业科学—基础兽医学]