地黄饮子含药脑脊液对受损PC12细胞β-淀粉样前体蛋白mRNA表达的干预  

PC12 Cells Injured by Amyloid-protein and Affected by the APP mRNA through Cerebrospinal Fluid with Drug-containing Dihuangyinzi

作　　者：何秀丽[1] 孟菲[1] 周妍妍[1] 谢宁[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2011年第4期26-28,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(30472128);高等学校博士学科点专项科研基金项目(20050228005)

摘　　要：目的:探讨地黄饮子含药脑脊液对β-淀粉样蛋白(Aβ25-35)所致PC12细胞损伤的β-淀粉样前体蛋白(APP)mRNA的表达及地黄饮子对其的干预作用。方法:运用中药脑脊液药理学方法把PC12细胞分为空白组,模型组,西药对照组,地黄饮子脑脊液低,中,高剂量组6组,分别加入正常培养液,正常脑脊液及含药脑脊液,应用实时定量PCR法检测受损PC12细胞β-淀粉样前体蛋白mRNA表达(用2-ΔΔC t表示,Ct为阈循环值,ΔΔCt=ΔCt各干预组-ΔCt空白组,ΔCt=CtAPP-CtGAPDH)。结果:地黄饮子能明显降低PC12细胞受β-淀粉样蛋白刺激时APP的过度反应,降低APP mRNA的表达,与模型组比较,差异有统计学意义(P<0.01)。结论:地黄饮子脑脊液可以抑制β-淀粉样蛋白损伤对PC12细胞的损伤。Objective：To discuss the cerebrospinal fluid with ancient prescriptions Dihuangyinzi which has a protective function by the APP mRNA on PC12 cells injured by β-amyloid protein（Aβ25-35）.Method： We divide PC12 cells into 6 groups-that is,the blank group,the model group,the control group of western medicine,and the cerebrospinal fluid groups with low-dose,medium-dose and high-dose of Dihuangyinzi with pharmacology method of cerebrospinal fluid containing Chinese medicine;and add normal culture medium,the normal cerebrospinal fluid and cerebrospinal fluid containing drugs respectively;APPmRNA expression in PC12 cells by real-time quantitative reverse transcription-PCR（expressed as 2-ΔΔCt,Ct as cycle threshold,ΔCt=Ct APP-Ct GAPDH,ΔΔCt=ΔCt invervention groups-ΔCt blank controls group）.Results： Decoction of Dihuangyinzi can significantly reduce the excessive reaction with the stimulation by the Aβ on PC12 cells and the Protein expression of APP with the model group;the difference has a statistics significance（P0.01）.Conclusion：Cerebrospinal fluid with Dihuangyinzi can inhibit the PC12 cells injury by Aβ.

关 键 词：Β-淀粉样前体蛋白 老年性痴呆 地黄饮子 PC12细胞 实时荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]