茯苓多糖的总糖含量及糖醛酸含量测定  

Determination of Total Carbohydrate and Uronic Acid Contents in Polysaccharides from Poria Cocos

作　　者：方东军[1] 赵润琴[1] 张晓娟[2] 

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2011年第4期42-44,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(D200928)

摘　　要：目的:建立茯苓多糖中总糖含量及糖醛酸含量的测定方法。方法:分别采用苯酚-硫酸比色法和咔唑-硫酸比色法进行操作,于分光光度计最大吸收波长处测定OD值。结果:95%醇沉茯苓多糖、70%醇沉茯苓多糖、30%醇沉茯苓多糖中总糖平均百分含量分别为67.96%、89.95%和79.16%;糖醛酸百分含量分别为8.00%、8.91%和8.65%。结论:总糖含量及糖醛酸含量的测定方法可以作为茯苓多糖的质量控制的方法。Objective： To establish a UV method for the determination of total carbohydrate and uronic acid contents in polysaccharides from Poria cocos wolf.Method： The contents of total carbohydrate and uronic acid were measured by phenol-sulfuric acid and carbazole-sulphuric acid methods,respectively.Result： The contents of three polysaccharide fractions required by 95%,70% and 30% alcohol sedimentation were 67.96%,89.95% and 79.16%,respectively,and the contents of uronic acids in P.cocos were 8.00%,8.91% and 8.65%.Conclusion： These methods can be used for the quality control of polysaccharides from P.cocos.

关 键 词：茯苓 多糖 苯酚-硫酸比色法 咔唑-硫酸比色法 

分 类 号：R284[医药卫生—中药学]