Establishment of novel embryonic stem(ES) cell lines from OG2/rtTA blastocysts  

作　　者：Hui Yao Yonghua Jiang YU Zhang Wenqiang Liu Bo Huang Xiaodong Wang Shaorong Gao 

机构地区：[1]College of Life Science, Beijing Normal University, Beijing 100875, China [2]National Institute of Biological Sciences, NIBS, Beijing 102206, China

出　　处：《Journal of Genetics and Genomics》2011年第7期289-295,共7页遗传学报（英文版）

基　　金：supported by the Ministry of Science and Technology of China(Nos.2010CB944900 and 2011CB964800)

摘　　要：Embryonic stem （ES） cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation. As the first discovered ES-specific transcription factor, Oct4 has been considered as the core pluripotency factor of ES cells. In the present study, we successfully established seven ES lines from the blastocysts collected from female OG2 （Oct4-GFP transgenic） mice, which have been crossed with male rtTA transgenic mice. The pluripotency of the ES cell lines can be visualized by the expression of Oct4-GFP under fluorescent microscopy and germ-line transmission capability has been further confirmed. More importantly, the presence of rtTA could induce transgene＇s expression with the help of doxycycline. Therefore, these ES cell lines provide an excellent tool to further discover novel factors affecting pluripotency and to investigate the molecular mechanism of reprogramming in defined transcription factors mediated nuclear reprogramming.Embryonic stem （ES） cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation. As the first discovered ES-specific transcription factor, Oct4 has been considered as the core pluripotency factor of ES cells. In the present study, we successfully established seven ES lines from the blastocysts collected from female OG2 （Oct4-GFP transgenic） mice, which have been crossed with male rtTA transgenic mice. The pluripotency of the ES cell lines can be visualized by the expression of Oct4-GFP under fluorescent microscopy and germ-line transmission capability has been further confirmed. More importantly, the presence of rtTA could induce transgene＇s expression with the help of doxycycline. Therefore, these ES cell lines provide an excellent tool to further discover novel factors affecting pluripotency and to investigate the molecular mechanism of reprogramming in defined transcription factors mediated nuclear reprogramming.

关 键 词：Embryonic stem （ES） cells OCT4 RTTA 

分 类 号：Q813[生物学—生物工程] Q78