疏肝健脾方药对非酒精性脂肪性肝病大鼠肝组织SREBP-1c mRNA及蛋白表达的影响  

Effect of Soothing Liver and Invigorating Spleen Recipes on SREBP-1c mRNA and Protein Expression in Hepatic Tissues of Rats with Non-alcoholic Fatty Liver Disease

作　　者：何秀敏[1] 杨钦河[1] 黎佩芳[2] 陈忠 冯高飞[1] 王文晶[1] 刘海涛[1] 张玉佩[1] 纪桂元[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632 [2]暨南大学附属第一医院,广东广州510632 [3]广州市白云区第二人民医院,广东广州510450

出　　处：《中药材》2011年第6期931-937,共7页Journal of Chinese Medicinal Materials

基　　金：广东省中医药强省科研资助课题(2010122);中央高校基本科研业务专项基金(21609415)

摘　　要：目的:观察疏肝健脾方药对非酒精性脂肪性肝病(NAFLD)大鼠肝组织SREBP-1c mRNA及蛋白表达的影响,并探讨其机制。方法:选雄性SPF级SD大鼠55只,随机分为模型组、正常对照组和3个中药干预组,分别为疏肝组、健脾组及合方组。除正常对照组外,各组采用脂肪乳剂灌胃8 w复制大鼠NAFLD模型,同时各药物干预组给予不同药物干预(10 mL/kg),正常对照组和模型组给予等体积蒸馏水灌胃。8 w后处死动物取肝组织,HE染色观察肝脏病理变化;RT-PCR方法检测肝组织SREBP-1c mRNA的表达;免疫组织化学方法检测肝组织SREBP-1c的蛋白表达水平和分布。结果:模型组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较正常对照组显著升高(P<0.01);各中药干预组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较模型组显著降低(P<0.05或P<0.01);疏肝组、健脾组大鼠肝组织SREBP-1c mRNA表达率较合方组显著降低(P<0.01)。病理观察显示疏肝、健脾组大鼠肝组织脂肪变最轻。结论:疏肝健脾方药可能是通过下调SREBP-1c mRNA和蛋白的表达来实现抗NAFLD作用,SREBP-1c可能是疏肝健脾方药抗NAFLD的作用靶点之一。Objective：To investigate the effects of soothing liver and invigorating spleen recipes on expression levels of Sterol Regulatory Element-binding Protein-1c（SREBP-1c） mRNA and SREBP-1c protein in hepatic tissue of rats with non-alcoholic fatty liver disease（NAFLD）.Methods：Fifty-five SD rats were randomized into 5 groups：normal control group,model group,soothing liver group,invigorating spleen group and combination group.Except the normal group,the rats in model group and other treatment groups were fed with high-fat emulsion to induce NAFLD.The treatment groups were administered with respective traditional chinese medicine,the normal group and model group received correspondence volume distilled water.After treatment for 8 weeks,the rats were executed to obtain the liver for observing hepatic pathological changes.Expression levels of SREBP-1c mRNA and SREBP-1c protein in hepatic tissues were assayed using reverse transcription polymerase chain reaction（RT-PCR）and immunohistochemistry respectively.Results：Compared with the normal group,the expression level of SREBP-1c mRNA and SREBP-1c protein in rat hepatic tissues of model group was significantly increased（P〈0.01）.Compared with the model group,the expression levels of SREBP-1c mRNA and SREBP-1c protein in the treated groups was decreased（P〈0.01,P〈0.05）.Specially,expression levels of SREBP-1c mRNA were the lowest in soothing liver group and invigorating spleen group and hepatic fatty changes were the slightest.Conclusion：Soothing liver and invigorating spleen recipes can inhibit hepatic SREBP-1c expression.That may be one of their therapeutic mechanisms.

关 键 词：非酒精性脂肪性肝病 疏肝健脾方药 固醇调节元件结合蛋白-1C 

分 类 号：R285.5[医药卫生—中药学]