不同量子点诱导L 929细胞氧化损伤及凋亡作用  

Effect of oxidative damage and apoptosis of different quantum dots on L929 cells

作　　者：王宜庆[1,2] 唐萌[1,2] 张婷[1,2] 陆敏玉[1,2] 杨扬[1,2] 张姗姗[1,2] 孔璐[1,2] 刘晓闰[1,2] 薛玉英[1,2] 张宇[2,3] 

机构地区：[1]环境医学工程教育部重点实验室东南大学公共卫生学院,江苏南京210009 [2]江苏省生物材料与器件重点实验室 [3]东南大学生物科学与医学工程学院

出　　处：《中国公共卫生》2011年第8期975-977,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(3067178230972504);国家重点基础研究"973计划"(2006CB705602);国家重大科学研究计划(2011CB933404)

摘　　要：目的探讨量子点CdSe及2种不同粒径的CdTe对L 929细胞增殖能力、氧化损伤和凋亡的影响。方法四甲基偶氮噻唑蓝(MTT)比色法检测3种量子点对L 929细胞增殖活力的影响;检测染毒后L 929细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-Px)和羟自由基(.OH)的含量及细胞凋亡情况。结果染毒24 h后半数抑制浓度(IC50)为:2.2 nm CdSe 27.14μg/mL,2 nm CdTe 28.28μg/mL,3.5 nm CdTe 79.30μg/mL;不同粒径的2种CdTe比较,14μg/mL剂量时,GSH-Px和.OH指标差异有统计学意义(P<0.05);3种量子点的高剂量组细胞凋亡率与对照组比较差异有统计学意义(P<0.05),不同粒径的2种CdTe比较,各剂量组差异均有统计学意义(P<0.05)。结论量子点可以抑制小鼠成纤维细胞增殖能力,并发生氧化损伤作用,诱导细胞凋亡,量子点的粒径大小为其引起细胞毒性的重要因素。Objective To study the effects of different quantum dots（QDs）（CdSe particles of 2.2 nm and CdTe particles of 2.0 and 3.5 nm in diameter） on proliferation activity,oxidative damage,and apoptosis of L929 cells.Methods Methyl thiazolyl tetrazolium（MTT） assay was applied to test the proliferation activities of L929 cells.Twenty-four hours after the treatment,malondialdehyde（MDA）,superoxide dismutase（SOD）,glutathione-peroxidase（GSH-Px）,and hydroxy radical（·OH） in the cells were determined.The apoptosis was detected with flow cytometry.Results The 50% inhibitory concentrations（IC50） was 27.14 μg/mL for 2.2 nm CdSe,28.28 μg/mL for 2.0 nm CdTe,and 79.30 μg/mL for 3.5nm CdTe,respectively.There were statistically significant differences in ·OH and GSH-Px contents between two groups with different size QDs exposure at the dose of 14 μg/mL（P0.05）.The apoptosis of each group at the dose of 14 μg/mL had significant difference compared with that of the control group and there also was a significant difference between the groups with QDs exposure of different size of the same dose.Conclusion QDs can inhibit the proliferation and induce oxidative damage and apoptosis of L929 cells and the size of QDs is an important influential factor of cytotoxicity.

关 键 词：量子点 CDSE L929细胞 氧化损伤 细胞凋亡 细胞毒性 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]