苯并芘对大鼠海马组织基因表达影响  

Gene chip screen for gene expression in rat hippocampus after chronic exposure to benzo(a)pyrene

作　　者：唐倩[1] 夏茵茵[1] 程淑群[1] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生学教研室,重庆400016

出　　处：《中国公共卫生》2011年第8期1002-1003,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671744)

摘　　要：目的采用基因芯片技术筛选苯并芘染毒大鼠海马组织差异表达基因,探讨苯并芘影响学习记忆能力机制。方法将18只雄性SD大鼠分为染毒组和对照组,每组9只;染毒结束后,基因芯片技术进行表达谱分析,从差异基因中选择差异表达明显的基因,再用real-time PCR技术对基因表达进行分析,与芯片分析结果进行比较。结果苯并芘染毒组和对照组间存在差异表达的基因有417条,其中高表达125条,低表达292条;real-time PCR检测方法验证其中8个学习记忆相关基因表达情况分别为:NR2A:7.361;adra1b:0.144;Gabra5:4.923;Egr2:0.257;Accn2:0.570;Pmch:30.909;Drd2:0.387;Drd1a:0.382。结论苯并芘通过不同程度地调节各种基因表达从而影响大鼠学习记忆能力;基因芯片和real-time PCR分析基因表达变化结果可信。Objective To investigate the effect of benzo（a）pyrene exposure on learning and memory of adult rats by determination of gene expression in the hippocampus.Methods Eighteen healthy male rats of clean class were randomly divided into toxic exposure and vehicle group.The gene chip technigue was adopted in gene expression profile analysis.The genes with significantly different expression were selected from differential genes and then analyzed with real-time PCR.Results Totally 417 genes exhibited differential expression between exposure group and vehicle group.The expression of 125 genes was up-regulated and 292 genes was down-regulated.The results of real-time PCR were coincided well with the cDNA microarray data.Conclusion The regulation of genes may be involved in the effect of benzo（a）pyrene on learning and memory in rat.

关 键 词：苯并芘 大鼠 海马组织 基因表达 

分 类 号：R135[医药卫生—劳动卫生]