抗氧化能力评价方法  

Methods to Determine Antioxidant Capacity

作　　者：马小媛[1,2] 钱卫平[1] 

机构地区：[1]东南大学生物科学与医学工程学院生物电子学国家重点实验室,南京210096 [2]东南大学化学化工学院,南京210096

出　　处：《化学进展》2011年第8期1737-1746,共10页Progress in Chemistry

基　　金：国家自然科学基金重大研究计划项目(No.90923010);国家重点基础研究发展计划(973)项目(No.2010CB933902);国家科技部国际合作项目(No.2008DFB50060);教育部高等学校博士学科点专项科研基金(No.20090092110026)资助

摘　　要：生物体系中的氧化与抗氧化系统失衡会导致氧化应激,被认为同诸多疾病的发病机理密切相关,具有抗氧化能力的物质能有效抵御氧化应激的有害损伤。本文介绍了氧化应激的有关概念,包括活性氧物种、抗氧化剂、氧化损伤等,并重点针对生物体内和体外的各种活性氧、活性氮物种清除能力检测方法以及本研究小组最新发展的利用金纳米壳复合材料和其他小组基于纳米材料的评价方法进行了综述与比较,包括化学原理、优缺点及实际应用等。简要分析了目前检测方法存在的问题并对未来标准化检测方法进行了展望。The imbalance between oxidants and antioxidants in biological systems may lead to oxidative stress, which is associated with the pathogenesis of several human diseases. The putative protective effects of antioxidants against these oxidative-induced reactions have received increasing attention. Here, the concept of oxidative stress is introduced, including reactive oxygen species, antioxidants, oxidative injury, etc. Furthermore, the methods most widely used for the determination of reactive oxygen species/reactive nitrogen species scavenging capacity as well as the newly developed evaluation assay using gold nanoshells composites by our research group and other methods based on nanomaterials are evaluated, presenting the general principles, recent applications, and their strengths and limitations. Finally, current problems and the prospect of the standardized analytical methods are proposed.

关 键 词：氧化应激 抗氧化 纳米材料 检测方法 

分 类 号：O652[理学—分析化学] Q503[理学—化学]