芦丁对罗丹明6G的荧光猝灭机理研究及其含量测定  

FLUORESCENCE QUENCHING MECHANISM OF RUTIN TO RHODAMINE 6G AND THE DETERMINATION OF RUTIN IN TABLETS

作　　者：刘晓慧[1] 吕庆銮 王怀友[3] 

机构地区：[1]山东师范大学实验室管理处,济南250014 [2]鲁南煤化工研究院,济宁272000 [3]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《化学分析计量》2011年第4期39-41,47,共4页Chemical Analysis And Meterage

摘　　要：根据Stern-Volmer方程和罗丹明6G吸收光谱的变化研究了芦丁对其荧光猝灭的类型及机理,结果表明,芦丁与罗丹明6G作用,形成一种稳定的复合物,而发生荧光静态猝灭,求得复合物的结合常数K=5.55×105L/mol,结合点数n=1.26。芦丁含量在0.92～137.8μg/mL范围内与F0/F成正比,检出限为0.90μg/mL。据此建立了一种灵敏、简便、快速测定芦丁的新方法,并用于芦丁片剂中芦丁含量的测定。The quenching mechanism and type of Rutin to Rhodamine 6G was studied based on the Stern - Volmer plot and the change of UV - Vis absorption spectrum of Rhodamine 6G. The result showed that Rutin reacted w.ith Rhodamine 6G to form a stable complex so as to fluorescence static quenching process. The binding constant K（ 5.55 ×10^5 L/mol） and the number of binding sites n（1.26） were obtained. A linear relationship was obtained between Fo/F and the concentration of Rutin in the range of 0.92 - 137.8 μg/mL. The detection limit was 0.90μg/mL. A simple, rapid and precise method for determining Rutin was established based on the fluorescence quenching principle. According to the method, the result obtained for the determination of Rutin in tablet samples accorded with that determined by UV- Vis spectrophotometry.

关 键 词：罗丹明6G 芦丁 荧光猝灭 Stern-Volmer方程 

分 类 号：O657.3[理学—分析化学]