元胡炮制前后UPLC指纹图谱研究  

UPLC fingerprint of crude and processed Corydalis Rhizoma

作　　者：张虹[1] 王麟[2] 陈相涛[1] 袁珂[1] 

机构地区：[1]浙江农林大学天然药物研发中心,浙江临安311300 [2]河南中医学院药学院,河南郑州450008

出　　处：《中成药》2011年第7期1093-1097,共5页Chinese Traditional Patent Medicine

基　　金：浙江省科技厅重点项目(2007C23024)

摘　　要：目的建立元胡炮制前后的UPLC指纹图谱。方法将元胡趁鲜切片,加醋后烘干得醋制元胡。元胡生、制品各10批,采用ACQUITY UPLCBEH C18色谱柱,PDA检测器,以0.2%甲酸水溶液-0.1%甲酸甲醇溶液为流动相进行梯度洗脱,选择色谱图出峰数目最多,各色谱峰分离度较好的297 nm为检测波长,柱温30℃,体积流量0.3 mL/min。结果以延胡索乙素作为参照,确定元胡生品有32个共有色谱峰,元胡制品有23个共有色谱峰。结论从整体上显示了生、制品的特征,炮制后多种未知成分的量下降或消失。建立的UPLC指纹图谱方法为元胡生、制品的质量控制提供有效手段。AIM To establish the fingerprint of crude and processed Corydalis Rhizoma by UPLC.METHODS Ten batches of sliced Corydalis Rhizoma were added vinegar and boiled to dryness as the processed along with another ten batches of unprocessed Corydalis Rhizoma for experiment.The UPLC analysis was carried out on an ACQUITY UPLC BEH C18 column,with a detector of PDA and the mobile phase consisting 0.2% formic acid water-0.1% formic acid methanol in gradient mode.The detection wavelength was set at 297 nm that more peaks can be detected.The column temperature was 30 ℃,and flow rate was 0.3 mL/min.RESULTS Thirty-two and twenty-three co-possessing peaks were selected as the fingerprint peaks of crude and processed Corydalis Rhizoma,respectively,based on the peak area of corydalis B as reference peak.CONCLUSION The method of UPLC fingerprint can display the feature of crude and processed Corydalis Rhizoma.After processing various chemical constituents are reduced and disappeare.It provides the effective means for quality control of crude and processed Corydalis Rhizoma.

关 键 词：UPLC 元胡 炮制 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]