高效液相色谱法测定穿龙骨刺片中薯蓣皂苷和淫羊藿苷  

Determination of dioscin and icariin in Chuanlong Guci Tablet by HPLC

作　　者：张伏军[1] 尹小玲[1] 李卿[2] 秦剑[2] 

机构地区：[1]重庆三峡中心医院药剂科,重庆404000 [2]重庆市中药研究院,重庆400065

出　　处：《中成药》2011年第7期1182-1185,共4页Chinese Traditional Patent Medicine

摘　　要：目的测定穿龙骨刺片(穿山龙,淫羊藿,狗脊,川牛膝,熟地黄,枸杞子)中薯蓣皂苷、淫羊藿苷。方法采用高效液相色谱法,色谱柱为C18柱,以乙腈-水为流动相,检测波长为203 nm(薯蓣皂苷)、270 nm(淫羊藿苷)。结果薯蓣皂苷进样量、淫羊藿苷进样量分别在0.56～5.60μg0、.24～2.40μg范围内与其峰面积积分值呈良好线性关系(r分别为0.999 9、0.999 8);平均回收率分别为98.23%、98.34%,RSD分别为0.88%(n=6)、0.62%(n=6)。结论 HPLC法快速、准确,可用于该制剂的质量控制。AIM To determine the contents of dioscin and icariin in Chuanlong Guci Tablet（Dioscoreae nipponicae Rhizoma,Epimedii Folium,Cibotii Rhizoma,Cyathulae Radix,Rehmanniae Radix Praeparata,Lycii Fructus）.METHODS HPLC method was adopted and a C18 column was used.The mobile phase was acetonitrile-water.The detection wavelengths were set at 203 nm（dioscin）、and 270 nm（icariin）.RESULTS The linear ranges were 0.56-5.60 μg（r=0.999 9） for dioscin,0.24-2.40 μg（r=0.999 8） for icariin,respectively.Their average recoveries were 98.23%（RSD was 0.88%）,98.34%（RSD was 0.62%）.CONCLUSION The method is quick and accurate,and it can be used for quality control of Chuanlong Guci Tablet.

关 键 词：薯蓣皂苷 淫羊藿苷 穿龙骨刺片 HPLC 

分 类 号：R927.2[医药卫生—药学]