霜制对苦杏仁中苦杏仁苷在大鼠尿液中的代谢与排泄影响  

Effect of defatted method on metabolism and excretion of bitter almond in rat urine

作　　者：付志玲[1] 郑晓晖[1] 房敏峰[1] 

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院,陕西西安710069

出　　处：《中成药》2011年第7期1202-1205,共4页Chinese Traditional Patent Medicine

基　　金：陕西省中医管理局基金资助项目(陕中医发20072507)

摘　　要：目的探讨霜制对苦杏仁中苦杏仁苷在大鼠尿液中的代谢与排泄影响。方法将SD大鼠分别注射苦杏仁苷、灌胃苦杏仁生品及霜制品水煎液,于不同时间段收集尿液,HPLC测定尿液中马尿酸、苦杏仁苷及其代谢产物野樱苷,LC-MSn鉴定代谢产物的结构。结果苦杏仁苷注射给药后主要以原型排泄,马尿酸质量分数无明显变化;苦杏仁生品与霜制品灌胃给药后主要以代谢产物野樱苷的形式排泄,马尿酸质量分数有显著增长,且霜制后野樱苷的排泄时间延迟。结论霜制对苦杏仁中苦杏仁苷的排泄有较大影响。AIM To explore the processing influence of removal of oil on metabolism and excretion of bitter almond in rat urine.METHODS Six SD rats were given the injection of amygdalin,other six SD rats took the perfusion of the extract of bitter almond,the third group of six SD rats took the extract of defatted bitter almond.Sample of these rats＇ urine was collected at various time intervals and contents of amygdalin,hippuric acid and prunasin analyzed on HPLC.LC-MS was applied to the structure analysis.RESULTS After drug administration,amygdain in prototype was found out in rat urine receiving injection of amygdain,hippuric acid content in rat urine continued without change.SD rats receiving perfusion excreted prunasin-metabolite of amygdain,and increased the excretion of hippuric acid.Experiment showed that the defatted bitter almond caused the excretion time interval of prunasin to be delayed.CONCLUSION The defatted processing may have an important effect on the excretion of amygdalin.

关 键 词：苦杏仁 霜制 代谢 高效液相色谱-质谱联用 

分 类 号：R285.5[医药卫生—中药学]