不同亚型哮喘患儿的IL-5、IL-17水平变化  

The variations of and clinical significance of interleukin-5 and Interleukin-17 levels in children with different subtype asthma

作　　者：李静[1] 檀卫平[1] 吴葆菁[1] 蓝丹[1] 麦贤弟[1] 范玲玲[1] 吴文英[1] 徐小彩[1] 

机构地区：[1]中山大学附属第二医院儿科,广东广州510120

出　　处：《中国热带医学》2011年第7期789-790,共2页China Tropical Medicine

基　　金：广东省科技计划项目(No.2007B030704004);广东省自然科学基金(No.9451008901003013)资助

摘　　要：目的探讨白细胞介素-5(IL-5)I、L-17在不同亚型中重度儿童哮喘发病中的作用。方法以酶联免疫吸附法(ELISA)检测45例不同亚型中重度哮喘患儿及20例正常儿童外周血单个核细胞(PBMC)在CD3单克隆抗体(0.2mg/L)及CD28单克隆抗体(1mg/L)刺激下分泌的IL-5I、L-17水平,了解IL-5I、L-17与儿童哮喘病情及气道炎症的关系。结果嗜酸细胞型哮喘(EA)组患儿PBMC表达IL-5水平(79.75±13.49)pg/ml显著高于非嗜酸细胞型哮喘(NEA)组(69.73±9.17)pg/ml及正常对照组(51.30±14.33)pg/ml(P<0.01),NEA组患儿PBMC表达IL-17水平(5684.1±884.74)pg/ml显著高于EA组(4938.1±465.58)pg/ml及正常对照组(2685.0±1081.03)pg/ml(P<0.01),慢性持续组IL-5、IL-17水平显著高于临床缓解组(P均<0.05)。结论中重度哮喘患儿外周血单个核细胞IL-5I、L-17表达水平升高,并与临床分期相关I,L-5在EA中的作用较NEA更为突出I,L-17在NEA中的作用较EA更为突出。Aim To investigate the effect of IL-5,IL-17 on the pathogenesis of moderate to severe children asthma in different subtypes. Methods ELISA was used to determine levels of IL-5 and IL-17 in peripheral blood mononuelear cells （PBMC）from 45 children with moderate to severe asthma in different subtypes and 20 healthy children as controls. PBMC were ,stimulated by anti-CD3 monoelonal antibody （OKT3）（0.2mg/L） and anti-CD28 monoelonal antibody （anti- CD28）（ling/L）. The relationship between IL-5,IL-17 and the disease severity and airway inflammation were also observed. Results The IL-5 level of EA group（79.75±13.49）pg/ml was significantly higher than that of NEA group（69.73±9.17） pg/ ml and control group（51.30±14.33） pg/ml（P〈0.01）. The IL-17 level of NEA group （5684.1±884.74）pg/ml was significantly higher than that of EA group （4938.1±465.58）pg/ml and control group （2685.0±1081.03）pg]ml（P〈0.01）. The IL-5 and IL- l7 level in chronic persistent group were significantly higher than those of clinical remission group （P〈0.05）. Conclusion The IL-5 and IL-17 levels in PBMC of moderate to severe asthmatic children rised,and were related to the disease severtiy.

关 键 词：哮喘 IL-5 L-17 

分 类 号：R521.1[医药卫生—内科学]