乙肝表面抗原检测确认试验方法的建立  

Establishment of a confirmation test for detcction of hepatitis B surface antigent

作　　者：刘荣静[1] 尤芳芳[2] 习浩[1] 吴晓蔓[1] 邓小燕[1] 

机构地区：[1]广州医学院第二附属医院检验科,广东广州510260 [2]广州医学院检验系,2005级学生广东广州510180

出　　处：《中国热带医学》2011年第7期815-817,共3页China Tropical Medicine

基　　金：广州医学院教育教学研究立项项目(No.2008-42)

摘　　要：目的探讨建立适合国产ELISA试剂检测乙型肝炎病毒表面抗原(HBsAg)阳性标本的确认试验方法。方法制备特异性抗-HBs(混合人血清抗-HBs),利用其可与临床样本中不同浓度的HBsAg发生中和反应来确定适合于实验的特异性抗-HBs浓度。根据临床需要确定最适反应时间。根据所测含不同浓度HBsAg临床标本的抑制率范围,选择最适抑制率阳性阈值。方法建立后,对临床标本进行检测,并与现有确认试剂盒比较,以检验实验方法的可靠性。结果试验方法所需特异性混合人血清抗-HBs浓度为2000IU/L,抑制率为50%,反应30min,对141例临床血清样本用确认试剂全部得到确认,对30份HBsAg阳性标本用本法与丽珠确认试剂作比对试验,二者结果一致,30份HBsAg阳性者均被确认。结论研究建立确认试验方法获得预期效果,适用于对HBsAg阳性标本的确认。Aim To set up a confirmation test for detection of samples positive for hepatitis B surface antigen by enzyme-linked immunosorbent assay （ELISA）. Methods The principle of neutralizing confirmatory test was used to confirm the samples positive for HBsAg. By comparing the different mixed human serum anti-HBs concentrations（5OOIU/L, 1 000 IU/L,2 000IU/L,3 000 IU/L,4 000IU/L）,reactive time （30min,60min） and inhibition rate the optimum threshold was chosen. The clinical samples were tested and the results were compared with the confirmed samples using national test kit . Results In the established confirmatory test,the anti-HBs concentration was 2 000IU/L,inhibition rate was 50% and 30 minute was the best reactive time. There 141 clinical samples were confirmed. Compared with the HBsAg confirmatory test kit porduced by livzon,30 positive samples were confirmed by both test kits and test reagent. Conclusion The established confirmatory test is suitable for clinical confirmation of samples positive for HBsAg.

关 键 词：乙肝炎病毒 HBSAG 确认试验 酶联免疫吸附测定法 弱反应性 

分 类 号：R373.21[医药卫生—病原生物学]