血清乙肝病毒外膜大蛋白检测在乙肝诊断治疗中的应用  

Application of detection of HBVlarge envelope protein in diagnosis and treatment of hepatitisb

作　　者：丁海明[1] 陈富[1] 潘婉仪[1] 

机构地区：[1]广东省中医院检验科,广东广州510105

出　　处：《中国热带医学》2011年第7期890-892,共3页China Tropical Medicine

摘　　要：目的通过检测乙肝病毒患者血清中乙肝病毒外膜大蛋白(HBV-LP)、HBV-DNA、乙肝前Sl蛋白(PreSlAg)以及乙肝两对半(HBVM),探讨HBV-LP与HBV-DNA、HBeAg、PreSlAg的关系及其在临床诊断、治疗中的应用价值。方法共检测174例乙肝病毒患者血清标本,HBV-LP、PreSlAg以及HBVM采用ELISA法进行检测,采用实时荧光定量PCR方法检测HBV-DNA。结果在乙肝病毒感染者血清中,HBV-LP、HBV-DNA和PreS1Ag的阳性率分别为83.3%、78.2%和65.5%,前两者与后者比较有统计学意义(P<0.05);随HBV-DNA拷贝数的增加,HBV-LP与PreS1Ag的阳性率随着增加,同时HBV-LP吸光度OD值也随着增加。对HBeAg阴性的血清,HBV-LP的阳性率(79.1%)明显高于PreS1Ag的阳性率(65.6%)(P<0.05)。结论 HBV-LP与HBV-DNA有良好的正相关性,HBV-LP与HBV-DNA的符合率高于PreSlAg,HBV-LP检测可以作为反映乙肝患者病毒复制的血清免疫学指标,也可作为判断HBV感染者体内病毒复制程度的可靠指标。Aim To detect serum hepatitis B virus large envelope protein （HBV-LP）,HBV-DNA,PreSlAg and HBVM from hepatitis B patients,and investigate the value in diagnosis and clinical analysis of hepatitis B virus infection. Methods There 174 serum samples from hepatitis B patients were detecrted, HBV-LP,PreSlAg and HBVM were detected with ELISA ,and serum HBV DNA was quantitively detected by real-time polymerase chain reaction （RT-PCR）. Results The positive rates of HBV-LP, HBV-DNA and PreslAg were 83.3%, 78.2%, 65.5%, showing significant difference in comparison of HBV-LP and HBV-DNA with PreslAg （P〈0.05）. The positive rates of HBV-LP and PreS1Ag were increased along with the increase of HBV copies. Meartwhile the HBV-LP absorbance OD was also increased. Positive rate of HBV-LP （79.1%） was signi/ieantly higher than PreSlAg （65.6%）in serum negative for HBeAg （P〈0.05）. Conclusions Serum HBV-LP level is closely con＇elated with the nunrber of HBV-DNA copies,coincldence between HBV-LP and HBV DNA is higher than PreSlAg, HBV-LP may be used as a new serological marker reflecting HBV replication and reliable indicator for judging HBV replication level.

关 键 词：乙肝病毒 病毒包膜蛋白 乙型肝炎 

分 类 号：R373.21[医药卫生—病原生物学]