四倍体湘鲫F_3 F_4、三倍体湘云鲫、湘云鲤及有关二倍体的DNA含量  

The Measurement of DNA Content of the Tetraploid F_3 F_4 Hybrids of Red Crucian Carp (♀)× Common Carp (♂) and Their Triploid Offspring and Other Related Diploid Fish

作　　者：刘少军[1] 冯浩[1] 刘筠[1] 周工建[1] 张轩杰[1] 罗琛[1] 何晓晓[1] 

机构地区：[1]湖南师范大学生命科学院,湖南长沙410081

出　　处：《湖南师范大学自然科学学报》1999年第4期61-68,共8页Journal of Natural Science of Hunan Normal University

基　　金：湖南省科委重点项目

摘　　要：用流式细胞仪测定湘鲫Ｆ２ 、湘云鲫、湘云鲤、湘鲫Ｆ３Ｆ４ 、湘江野鲤、红鲫、白鲫的红细胞、肌肉细胞中的ＤＮＡ 含量。结果表明：湘鲫Ｆ２ 的ＤＮＡ 含量与白鲫、红鲫、湘江野鲤的ＤＮＡ 含量很接近，其ＤＮＡ含量比与１∶１ 的比例没有显著差异（Ｐ＞ ０－０５） ；湘云鲫和湘云鲤的ＤＮＡ含量都是湘江野鲤的ＤＮＡ含量的１－５ 倍，其比值与１－５∶１ 的比例没有显著的差异（Ｐ＞ ０－０５） ；湘鲫Ｆ３ 和Ｆ４ 的ＤＮＡ含量都是湘江野鲤或湘鲫Ｆ２ 的ＤＮＡ含量的２ 倍，其比值与２∶１ 的比例没有显著的差异（Ｐ＞ ０－０５） ．上述结果提供了湘鲫Ｆ３ 、Ｆ４ 代为四倍体和湘云鲫及湘云鲤为三倍体的证据。The DNA content of red blood cells of the tetraploid F 3 F 4 hybrids of red crucian carp (♀)× common carp (♂) and their triploid offspring,diploid common carp (Cyprinus carpio),red crucian carp (Carassium auratus red var.),Japanese crucian carp (Carassium auratus cuvieri) and F 2 hybrid was measured by the instrument of the flow cytometer.The DNA content of muscle cells of F 3 hybrid was also measured by the same instrument.The results showed that the DNA content of F 3 F 4 hybrids was about two times of that of the diploid common carp(P>0 05).The DNA content of the triploid fish produced by mating F 3 F 4 hybrid (♂) respectively with Japanes crucian carp (♀) and Xin guo red common carp (♀) was about 1 5 times larger than that of the diploid common carp (P>0 05).F 2 hybrid,Japanese crucian carp,red crucian carp and common carp had the approximate value of the DNA content (P>0 05).The above results provided further evidence that F 3 F 4 hybrids were tetraploids and F 2 hybrid was diploid.Those data also confirmed that the fish produced by mating F 3 F 4 hybrids respectively with the Japanese crucian carp and Xin guo red carp were triploids.

关 键 词：DNA 四倍体 三倍体 二倍体 湘鲫 湘云鲫 湘云鲤 

分 类 号：Q959.468[生物学—动物学]