DNA分子力学性质的测量  

Measuring the elastic behavior of DNA

作　　者：冉诗勇[1] 王艳伟[1] 杨光参[1] 

机构地区：[1]温州大学物理与电子信息工程学院物理实验中心,浙江温州325035

出　　处：《物理实验》2011年第7期1-4,共4页Physics Experimentation

基　　金：浙江省自然科学基金资助项目(No.Y4110357);温州大学研究生教改项目(No.YJG200909)

摘　　要：利用单分子实验技术测量了DNA分子的力学性质.通过生化手段将单根DNA分子连在顺磁小球与玻璃侧壁之间,利用磁铁对顺磁球施加力进而拉伸DNA.图像分析得到小球运动的轨迹以及DNA的长度,利用均分定理可以求得施加的外力.测量发现在外力作用下DNA分子的力学反应与高分子物理学的蠕虫状链模型符合良好.The experiment on measuring the elastic behavior of DNA molecule using single molecule method was presented. A single DNA molecule was linked between a paramagnetic bead and the sidewall of a glass slide using biochemical method. The trajectory of the bead and the end-to-end length of the DNA molecule were obtained through image analysis. Then, the applied force was calculated according to equipartition theorem. It was found that the mechanic responses to forces agreed with worm-like-chain model well.

关 键 词：DNA 力学性质 单分子 均分定理 

分 类 号：Q66[生物学—生物物理学] Q6-33