苯甲酸雌二醇对大肠杆菌RecQ解旋酶生物学活性的影响  

Effects of Estradiol Benzoate on the Bioactivity of Escherichia coli RecQ Helicase

作　　者：段丽霞[1] 许厚强[1] 陈祥[1,2,3] 骆衡[1,3] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州省动物遗传育种与繁殖人才基地,贵阳550025 [2]贵州大学教育部绿色农药与农业生物工程重点实验室,贵阳550025 [3]贵州大学贵州山地农业病虫害重点实验室,贵阳550025

出　　处：《中国药学杂志》2011年第15期1146-1150,共5页Chinese Pharmaceutical Journal

基　　金：国家重点基础研究发展计划973计划资助项目(2010CB534912);教育部博士点基金资助项目(200806570003);贵州省优秀人才省长资金资助项目(200822);贵州省研究生创新基金资助项目(省研农2010004)

摘　　要：目的研究体外苯甲酸雌二醇对大肠杆菌RecQ解旋酶生物学活性的影响及作用机制。方法运用荧光偏振技术和ATPase检测技术分析苯甲酸雌二醇对大肠杆菌RecQ解旋酶活性、DNA结合活性和ATPase活性的影响。结果苯甲酸雌二醇对RecQ解旋酶活性、DNA结合活性及ATPase活性均有弱抑制作用,对RecQ解旋酶活性抑制常数Ki为5×10-3 nmol.L-1,对dsDNA和ssDNA结合活性的Ki分别为5×10-2和0.5 nmol.L-1。结论在体外苯甲酸雌二醇对大肠杆菌RecQ解旋酶生物学活性具有弱抑制作用,二者至少有两个作用位点。OBJECTIVE To study the effects of estradiol benzoate on the bioactivity of Escherichia coli RecQ helicase in vitro and the molecular mechanisms. METHODS The helicase activity, DNA binding activity, and ATPase activity of E. coli RecQ helicase were determined by fluorescence polarization and PiColorLock Gold reagent. RESULTS The helicase activity, DNA binding activity and ATPase activity were weakly inhibited by estradiol benzoate. The inhibition constants （Ki ） for the helicase activity was 5 x 10-3 nmol· L^-1. The Ki for DNA binding activity was 5 × 10^-2 nmol· L^-1when the substrate was dsDNA, and 0. 5 nmol· L^-1 when the substrate was ssDNA. CONCLUSION The biological activity of E. coli RecQ helicase is weakly inhibited by estradiol benzoate in vitro. There are at least two binding sites between them.

关 键 词：苯甲酸雌二醇 RecQ解旋酶 解旋酶活性 DNA结合活性 ATPASE活性 

分 类 号：Q557[生物学—生物化学]