甘草中50种有机氯及拟除虫菊酯农药残留量的分析方法研究  

Study on Analysis Method for 50 Organochlorine and Pyrethroid Pesticides Residues in Glycyrrhiza

作　　者：黄晓会[1,2] 薛健[1] 吴晓波[1] 佟鹤芳[1] 庞作正[1] 薛雯[1] 程猷[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100193 [2]宁夏医科大学研究生学院,银川750004

出　　处：《中国药学杂志》2011年第15期1204-1208,共5页Chinese Pharmaceutical Journal

基　　金：国家重大创新药专项资助项目(2009ZX09502-027;2009ZX09308-006;2009ZX09308-001-1-5)

摘　　要：目的建立甘草中50种有机氯及拟除虫菊酯农药残留的气相测定方法。方法以乙腈为提取剂,提取液用弗罗里硅土柱净化,乙酸乙酯-正己烷(15∶85)洗脱,采用HP-5与DB1701毛细管色谱柱双柱定性,以HP-5毛细管柱定量,GC-ECD检测器进行检测。结果样品3水平添加时的50种有机氯及拟除虫菊酯农药平均回收率在70.3%～115.6%,相对标准偏差在1.1%～13.1%,符合农药残留分析的要求。结论本方法重复性及净化效果好,可用于甘草中50种有机氯及拟除虫菊酯类农药残留量检测。OBJECTIVE To establish a gas chromatography method for the determination of 50 organochlorine and pyrethroid residues in Glycyrrhiza products. METHODS Oranochlorine and pyrethroid pesticides were extracted from Glycyrrhiza uralensis Fisch. with acetonitrile. Then the extracts were cleaned up by alumina neutral-florisil column and eluted by mixed solvents of ethyl acetate and hexane （15： 85）. The extract was separated by HP- 5 and DB1701 capillary dual-column and detected by electron-capture detector. RESULTS The average recoveries and RSDs ranged from 72. 9% to 115.8% and 1.1% to 13.1%, respecttivily, at 3 levels of spiked mixed organochlorine and pyrethroid. CONCLUSION The method has good separation and repealability and can be used in determination of organochlorine and pyrethroid pesticide residues in Licorice products.

关 键 词：甘草 毛细管气相色谱 有机氯农药 拟除虫菊酯农药 

分 类 号：R917[医药卫生—药物分析学]