HPLC同时测定麦冬药材中3种黄酮类成分的含量  

Simultaneous Determination of Contents of Three Flavonoid Ingredients in Radix Ophiopogonis by HPLC

作　　者：贾诚[1,2] 叶正良[2] 姜秀晶[1] 周大铮[2] 李德坤[2] 

机构地区：[1]天津中医药大学中药学院,天津300193 [2]天津天士力之骄药业有限公司,天津300402

出　　处：《中国药学杂志》2011年第15期1209-1211,共3页Chinese Pharmaceutical Journal

基　　金："国家重大新药创制"科技重大专项(2010ZX09502-004);科技创新专项资金(08FDZDSH01404)

摘　　要：目的测定麦冬药材中甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A的含量,为麦冬药材的质量控制提供科学依据。方法采用Waters Symmetry C18柱(4.6 mm×250 mm,5μm),乙腈-0.05%磷酸梯度洗脱,流速为1.0 mL.min-1,检测波长296 nm,柱温30℃。结果甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A分别在9.68～96.8、4.96～49.6、4.18～41.8μg.mL-1内具有良好的线性关系,平均回收率(n=6)分别为100.1%(RSD 1.03%),99.5%(RSD 1.35%)和100.2%(RSD 1.16%)。结论该方法快速、准确,可用于麦冬药材中甲基麦冬黄烷酮A、甲基麦冬黄烷酮B和6-醛基异麦冬黄烷酮A的含量测定。OBJECTIVE To determine the contents of methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis. METHODS Waters Symmetry C ls column （4. 6 mm x 250 mm, 5 μm） was used with phosphoric acid （0. 05%, v/v） and acetonitrile as the mobile phase. Stepwise gradient elution was adopted. The flow rate was 1.0 mL·min^-1, the detection wavelength was 296 nm, and the column temperature was maintained at 30 ℃. RESULTS The calibration curves showed good linearity within the range of 9. 68 -96. 8, 4. 96 -49. 6 and 4. 18 -41.8 μg ·min^-1for methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A, respectively. The mean recoveries （ n = 6 ） were 100. 1% （ RSD 1.03% ）, 99. 5% （RSD 1.35% ） and 100. 2% （ RSD 1.16% ）, respectively. CONCLUSION This method is simple and accurate. And it can be used for the determination of methylophiopogonanone A, methylophiopogonanone B and 6-aldehydo-isoophiopogonone A in Radix Ophiopogonis.

关 键 词：麦冬 甲基麦冬黄烷酮A 甲基麦冬黄烷酮B 6-醛基异麦冬黄烷酮A HPLC 

分 类 号：R283[医药卫生—中药学]