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作 者:宋潇[1] 戚爱棣[1] 王跃飞[1] 经雅昆[1] 柴欣[1] 刘亚男[1]
机构地区:[1]天津中医药大学中医药研究院天津市现代中药重点实验室,天津300193
出 处:《中国中药杂志》2011年第15期2071-2075,共5页China Journal of Chinese Materia Medica
基 金:天津市高等学校科技发展基金计划项目(20090223);天津市科技支撑计划项目(10ZCKFSY09100)
摘 要:目的:采用高效液相色谱法分析补骨脂不同炮制品中7种成分的含量,研究炮制方法对补骨脂中糖苷、香豆素、黄酮、单萜酚4类成分的影响。方法:采用ACUITY C18色谱柱(3.0 mm×100 mm,1.7μm),流速0.4 mL.min-1,柱温50℃,检测波长246 nm,流动相乙腈-0.1%甲酸水,梯度洗脱。结果:25 min内补骨脂苷、异补骨脂苷(糖苷),补骨脂素、异补骨脂素、补骨脂定(香豆素),补骨脂二氢黄酮(黄酮),补骨脂酚(单萜酚)分离良好。在相应浓度范围内7种化合物的线性关系良好,r均在0.999 3以上;精密度RSD≤1.5%;平均加样回收率为99.2%~106%。结论:该方法快速、准确,适用于补骨脂不同炮制品中7种(4类)化学成分的定量分析。在4种炮制方法中,雷公炮制法较其他3种方法明显降低糖苷、黄酮、单萜酚类成分的含量,明显升高香豆素类成分补骨脂素、异补骨脂素的含量。Objedtve: To established an efficient method for simultaneous quantification of 7 compounds belonging to 4 chemi- cal types in Psoralea corylifolia processed by different methods, and to elucidate variations of 4 kinds of compounds in different pro- cessed P. corylifolia. Method: The chromatographic separation was performed on an ACUITY C18 column using acetonitrile and 0. 1% formic acid aqueous solution in the gradient elution at 0. 4 mL·min^-1, Detection wavelength was set at 246 nm. Column temperature was fixed at 50℃. Result: The 4 kinds of compounds including psoralenoside, isopsoralenoside ( benzofuran glycosides), psoralen, isopsoralen, psoralidin (coumarins), bavachin (flavonoids), and bakuchiol (meroterpenes) were separated in 25 min. The correla- tion coefficients of those compounds were over 0, 9993 in the tested range. The intra- and inter-day precisions were below 1.5%. The average recoveries ranged from 99. 2% to 106%. Conclusion: This method is simple, rapid and accurate, which can be used for the determination of the 4 kinds of compounds in different processed P. corylifolia. In P. corylifolia processed by different methods, the contents of benzofuran glycosides, flavonoids, and meroterpenes in P. corylifolia processed by Leigong' s method decrease obviously, while the contents of psoralen and isopsoralen increase significantly.
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