鱼腥草挥发油与甲基正壬酮在大鼠体内代谢产物“网通性”的研究  

Study on network compatibility of metabolisms in vivo rat for volatile oil in Houttuyniae Herba and 2-undecanone

作　　者：邓凯文[1] 贺福元[2,3,4] 石继连[2,3] 刘文龙[2,3] 邹欢[2,3] 邱云[2,3] 陈锋[2,3] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学药学院,湖南长沙410208 [3]湖南中医药大学中药药性与药效国家中医药管理局重点实验室,湖南长沙410208 [4]湖南中医药大学现代中药制剂制备技术与评价实验室,湖南长沙410208

出　　处：《中国中药杂志》2011年第15期2076-2083,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073142);湖南省教育厅重点项目(09A068)

摘　　要：目的:研究大鼠口服或注射鱼腥草挥发油与甲基正壬酮后血液中的主要代谢产物,验证"网通性"假说,为揭示中药多成分的代谢规律奠定理论基础。方法:采用GC-MS联用技术对鱼腥草挥发油、大鼠口服或注射鱼腥草挥发油与甲基正壬酮后血液中的代谢产物进行定性分析,比较2种药物、2种给药方式代谢产物的异同。结果:原鱼腥草挥发油共45种成分,大鼠口服鱼腥草挥发油与甲基正壬酮后血液中分别出现了53,69种代谢产物,大鼠注射鱼腥草挥发油与甲基正壬酮后,血液中分别出现了52,42种代谢产物;单成分与多成分给药,口服与注射给药,均产生了相同相似的代谢产物,α-蒎烯、β-月桂烯为各组共有的代谢产物,其中鱼腥草挥发油口服与注射血样出现共有代谢产物18,13个,分别占34%,25%;甲基正壬酮口服与注射血样出现共有代谢产物17,15个,占24.6%,35.7%,平均为29.8%,近1/3的代谢产物为共同代谢产物。结论:鱼腥草挥发油及甲基正壬酮在大鼠体内的代谢存在网通性,中药各成分之间的代谢与原药材成分代谢途径相关。Objective： To study on the differences of metabolites in the blood of rats after injection or oral administration of volatile oil in Houttuyniae Herba and of 2-undecanone in order to validate hypothesis of network compatability and to establish the theo- retical foundation of elucidating metabolic mechanism for multiple constituents in Chinese Material. Method： A gas chromatography- mass spectrometry method was employed for qualitative determination of the metabolites in blood of rats by injection or oral administra- tion of volatile oil in Houttuyniae Herba and of 2-undecanone, which were compared with the differences between binary two drugs as well as two administration. Result： There were 45 kinds of ingredients in volatile oil for original materials for Houttuyniae Herba, and 53, 69 kinds of metabolites were detected in blood of rats for oral administration of volatile oil in Houttuyniae Herba, and 2-undecan- one, respectively, whereas 52, 42 kinds of metabolites were detected for injection administration. Although there were various consti- tutes, i. e. single versus multiple ingredients, administrated by various ways, i. e. oral versus injection, the metabolites were so simi- lar with each manners, in which α-pinene and β-myrcene occurred in two constitute forms and two administrations, while there were 18, 13 kinds of ingredients for oral or injection administration of volatile oil in Houttuyniae Herba, i. e. 34%, 25% of total ingredi- ents, respectively, and 17, 15 kinds of ingredients, i. e. 24.6%, 35.7% for that of , 2-undecanone, respectively, on average of 29. 8% that suggested one of third as co-metabolites. Conclusion： There are network compatibility existed in metabolism of the hout- tuynia volatile oil in rats, and similar metabolic pathways in accordance with original plant, among their ingredients no matter alone or multiple constitute forms and oral or injection administration.

关 键 词：鱼腥草挥发油 甲基正壬酮 中药代谢 网通性 网通虹势 口服 注射 

分 类 号：R284[医药卫生—中药学]