复元胶囊及其主要成分淫羊藿苷、三七皂苷R_1治疗骨关节炎的分子机制研究  

Study of molecular mechanisms of Fuyuan capsule,icariin and arasaponin R_1 in treatment of osteoarthritis

作　　者：张文亮[1,2] 李荣亨[1] 王淑美[3] 周小莉[1] 钟玉[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]重庆电力医院,重庆400030 [3]重庆医科大学中医药学院,重庆401331

出　　处：《中国中药杂志》2011年第15期2113-2117,共5页China Journal of Chinese Materia Medica

基　　金：重庆市卫生局课题([2004]12号B-55)

摘　　要：目的:从尿激酶型纤溶酶原激活物(uPA)系统研究复元胶囊含药血清及其主要成分淫羊藿苷、三七皂苷R1治疗骨关节炎的分子机制。方法:原代培养兔软骨细胞,TNF-α诱导软骨细胞损伤,分为7组:空白组、模型组(TNF-α10μg.L-1组)、盐酸氨基葡萄糖25 g.L-1组、20%复元胶囊含药血清组、淫羊藿苷12.5 mg.L-1组、三七皂苷R1 125 mg.L-1组、淫羊三七组(12.5,125 mg.L-1),RT-PCR检测各组uPA,NF-κB(P65)mRNA的含量,EMSA检测NF-κB(P65)与DNA结合的活性,Western检测IκBα水平。结果:复元胶囊含药血清及其主要成分淫羊藿苷、三七皂苷R1均可显著降低uPA,NF-κB mRNA表达量,降低NF-κB(P65)与DNA结合的活性,升高IκBα水平,与TNF-α组比较,统计学有显著差异(P<0.01),各给药组间无明显差异。结论:复元胶囊含药血清及其主要成分淫羊藿苷、三七皂苷R1可通过降低NF-κB(P65)的活性,升高IκBα水平,从而降低uPA的表达,保护软骨细胞免受炎症因子的损伤。Objective： To study the molecular mechanisms of Fuyuan capsule serum containing, icariin and arasaponin R1 in the treatment of osteoarthritis from the urokinase-type plasminogen activator （uPA） system. Method： Chondrocytes were isolated, cul- tured and identified using type I1 collagens immunostaining. After stimulating with TNF-α 10 μg · L^-1, 1 h, then the ehondrocytes were treatment with glucosamine hydrochloride 25 g · L-l , 20% Fuyuan capsule serum c0ntaining, ieariin 12. 5 mg · L^-1 , arasaponin Rl 125 mg·L^-1 , icariin 12. 5 mg · L^-1 ＋ arasaponin R1125 mg · L^-1. After 2 h, expression of uPA and nuclear factor kappa B （NF-κB P65 ） mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）, the activities of NF-κB （P65） combine DNA were determined by electrophoretie mobility shift assays （EMSA）, IκBα were detected by Western blotting. Result： Fuyuan capsule, ieariin and arasaponin R1 could significantly reduce NF-κB（ P65 ） activities and uPA mRNA expression, and increase expression of IκBα（P 〈0. 01 ）, but no significant difference between all treatment groups. Condusion： Fuyuan capsule and its two main active ingredients, ieariin and arasaponin R1, could protect chondrocytes from damage through reducing the NFκKB（P65） activities, in- creasing the express of IκBα and then reducing uPA of chondrocytes.

关 键 词：复元胶囊 淫羊藿苷 三七皂苷R1 软骨细胞 NF—κB(P65) IΚBΑ UPA 

分 类 号：R285[医药卫生—中药学]