丹参酮Ⅱ_A诱导人鼻咽癌CNE细胞凋亡  

Apoptosis inducing effect of tanshinone Ⅱ_A on human nasopharyngeal carcinoma CNE cells

作　　者：戴支凯[1,2] 黄大林[3] 石京山[2] 余丽梅 吴芹[2] 徐庆[1] 

机构地区：[1]桂林医学院药理学教研室,广西桂林541004 [2]贵州省基础药理重点实验室,贵州遵义563003 [3]桂林医学院微生物学教研室,广西桂林541001 [4]贵州省细胞工程重点实验室,贵州遵义563003

出　　处：《中国中药杂志》2011年第15期2129-2133,共5页China Journal of Chinese Materia Medica

摘　　要：目的:探讨丹参酮ⅡA(tanshinoneⅡA,TanⅡA)抗人鼻咽癌CNE作用及其可能作用机制。方法:通过细胞形态学观察、生长曲线绘制、MTT检测以及克隆实验观察TanⅡA对CNE细胞增殖的影响;应用Hoechest33258和PI双染法观察TanⅡA对CNE细胞凋亡的影响;采用荧光分光光度计检测TanⅡA对CNE细胞细胞内钙及线粒体膜电位的影响;RT-PCR检测TanⅡA对CNE细胞Bad,MT-1A mRNA表达的影响。结果:TanⅡA能抑制CNE细胞增殖,且随TanⅡA剂量的增加和作用时间的延长而增强,TanⅡA作用CNE细胞24,48,72 h的IC50分别为45.7,24.8,3.3 mg.L-1。TanⅡA作用CNE细胞24 h后,CNE细胞出现染色质聚集等典型的凋亡形态学改变,且随TanⅡA剂量的增加,CNE细胞凋亡百分率逐渐增大。TanⅡA作用后,CNE细胞的细胞内钙升高、线粒体膜电位降低、Bad mRNA表达增加、MT-1A mRNA表达上调。结论:TanⅡA具有抗CNE作用,其诱导细胞凋亡可能与钙依赖性通路和MT-1A表达上调有关。Objective： To investigate anticancer effect and potential mechanism of tanshinone ⅡA （TanⅡA） on human naso- pharyngeal carcinoma cell line CNE cells. Method： Antiproliferative effect of Tan ⅡA on CNE cells was evaluated by morphological examination, cell growth curves, colonial assay and MTT assay. Apoptosis detection was carried out using Hoechest33258 and PI doub- le-dyeing method. Intracellular Ca2＋ concentration and mitochondria membrane potential were detected by fluorospeetrophotometer. Bad and MT-1A transcript analysis in CNE cells was analyzed by real-time reverse transcriptase-polymerase chain reaction （RT-PCR）. Result： Tan ⅡA could inhibit CNE cells proliferation in dose- and time-dependent manner. 50% inhibiting concentration of Tan ⅡA on CNE cells in 24,48,72 h was 45.7,24. 8,3.3 mg · L-1, respectively. Typical apoptotic morphology such as chromatin agtvegation was observed in CNE cells with Tan ⅡA treated for 24 h, and the apoptotic inducing effect was in a dose-dependant manner. After treated with TanⅡA,intracellular Ca2 ＋ concentration of CNE cells was increased, mitochondria membrane potential of the cells was de- creased ,relative mRNA level of Bad and MT-1A was up-regulated. Conclusion： Tan ⅡAhad anticancer effect on CNE cells through apoptosis via calcineurin-dependent pathway and MT-1A downregulation.

关 键 词：丹参酮ⅡA 肿瘤 人鼻咽癌CNE细胞 凋亡 钙依赖性通路 MT-1A 

分 类 号：R285[医药卫生—中药学]