中药复方小续命汤组分活性评价及抗脑缺血有效成分组制备  

Activity evaluation of components and preparation of effective components group of Xiaoxuming decoction for anti-cerebral ischemic

作　　者：王月华[1] 秦海林[1] 贺晓丽[1] 杜冠华[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《中国中药杂志》2011年第15期2140-2144,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金重点项目(30630073)

摘　　要：目的:在前期的研究基础上,通过化学分析和药理活性分析相结合的方法,制备小续命汤抗脑缺血有效成分组。方法:DPPH法测定样品对自由基的清除作用;MTT法测定细胞存活率,观察样品对过氧化氢损伤、谷氨酸损伤及低氧损伤的保护作用;TBA法测定线粒体脂质过氧化。结果:综合分析多靶点活性,40%乙醇洗脱物和中间层的活性最强。结论:40%乙醇洗脱物和中间层按比例混合为小续命汤抗脑缺血有效成分组。Objective： On basis of preliminary studies, to prepare the effeetive^components group of Xiaoxunfing decoction for anti-ischemie by combining chemical analysis with pharmacological activity screening. Method： Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay ; TBA meth- od was used to determine mitochondrial lipid peroxidation. Result： Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest. Conclusion： 40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.

关 键 词：小续命汤 有效成分组 脑缺血 

分 类 号：R285[医药卫生—中药学]