大鲵皮肤分泌液中抗菌肽的鉴定及生物活性研究  

Characterization and bioactivity of antimicrobial peptides from the skin secretions of the Andrias davidianus

作　　者：王利锋[1] 李学英[1] 王大忠[1,2] 

机构地区：[1]遵义医学院细胞生物学与遗传学教研室,贵州遵义563003 [2]铜仁学院,贵州铜仁554300

出　　处：《中国生化药物杂志》2011年第4期269-272,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：贵州省教育厅自然科学研究重点项目(黔教科2007039);贵州省高层次人才科研条件特助经费(TZJF-2007049)

摘　　要：目的鉴定大鲵皮肤分泌液中抗菌肽,研究其部分生物活性。方法 5%醋酸浸提和Sephadex G-50、G-25凝胶过滤色谱等方法分离纯化抗菌肽;采用抑菌圈法检测抗菌活性,Tricine-SDS-PAGE电泳和等电聚焦电泳鉴定其抗菌活性成份。结果大鲵皮肤分泌液中含有抗菌活性物质。对革兰阴性菌、革兰阳性菌和真菌均具有较强的抗菌活性;电泳检测显示该小分子多肽相对分子质量约为4 300,具有较强的碱性。结论首次从大鲵皮肤分泌液中分离纯化到一种抗菌肽,此抗菌肽可能是一个具有较强阳离子特征的碱性肽。Purpose To characterize the antimicrobial peptides from the skin secretions of the Andrias davidianus and to study its bioactivity.Methods This antimicrobial peptide was purified by 5%acetic acid extraction and Sephadex G-50,G-25 gel filtration chromatography.The antimicrobial ability was characterized by inhibiting zone determination.The antimicrobial active substance was identified by tricine-SDS-PAGE electrophoresis and isoelectric focusing（IEF） electrophoresis.Results The skin secretions of the Andrias davidianus contain antibiotic active substance.It strongly inhibited Gram-negative,Gram-positive bacteria and fungus.The electrophoresis results showed that its molecular weight was about 4 300 with a strong alkaline property.Conclusion An antibacterial peptide was isolated from the skin secretions of the Andrias davidianus for the first time and this antimicrobial peptide may be a strong feature of the basic peptide cation.

关 键 词：大鲵 皮肤 抗菌肽 分离纯化 

分 类 号：R282.74[医药卫生—中药学] R961[医药卫生—中医学]