广东兰花两种主要病毒的检测  

Detection of two major viruses in orchids in Guangdong

作　　者：吴竹妍[1] 黎园[1] 何晓盈[1] 童诗涵[1] 孙辉[2] 向梅梅[2] 饶雪琴[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]仲恺农业工程学院,广东广州510225

出　　处：《广东农业科学》2011年第11期14-16,F0002,共4页Guangdong Agricultural Sciences

基　　金：广东省现代农业产业技术体系建设创新团队项目(B3091236)

摘　　要：采用双抗夹心酶联免疫吸附法(DAS-ELISA)对44个兰花病样分别检测建兰花叶病毒(Cymbidium mosaic virus,CyMV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV),结果显示,有59.1%的兰花检出CyMV,36.4%的兰花检出ORSV,22.7%的兰花复合感染了CyMV和ORSV。采用一步逆转录聚合酶链式反应(RT-PCR)对24个兰花病样进行检测,结果有83.3%的兰花检出CyMV,75%的兰花检出ORSV,66.7%的兰花复合感染了CyMV和ORSV,可见RT-PCR检测灵敏度高于ELISA。Cymbidium mosaic virus（CyMV） and Odontoglossum ringspot virus （ORSV） were evaluated by DAS-ELISA respectively in 44 diseased orchid samples collected from Guangdong province, the resohs showed that CyMV and ORSV were detected in 59.1% and 36.4% respectively, mixed infections with CyMV and ORSV were detected in 22.7% of the 44 diseased orchid samples. CyMV and ORSV were evaluated by one step RT-PCR respectively in 24 diseased orchid samples collected from Guangdong province, the results showed that CyMV and ORSV were detected in 83.3% and 75% respectively, mixed infections with CyMV and ORSV were detected in 66.7% of the 24 diseased orchid samples. These results suggested that the sensitivity of RT-PCR method is higher than that of DAS-ELISA method.

关 键 词：兰花 建兰花叶病毒 齿兰环斑病毒 DAS—ELISA 一步法RT—PCR 检测 

分 类 号：S432.41[农业科学—植物病理学]