不同猪种乳铁蛋白基因启动子区单核苷酸多态性分析  

Single nucleotide polymorphism of the promoter of lactoferrin gene in pig

作　　者：刘晓静[1] 姚志文[1] 李兆能[1] 何必宏[1] 吴琳兰[1] 高萍[1] 李加琪[1] 张爱玲[1] 

机构地区：[1]华南农业大学动物科学学院/广东省农业动物基因组学与分子育种重点实验室,广东广州510642

出　　处：《广东农业科学》2011年第11期151-153,共3页Guangdong Agricultural Sciences

基　　金：现代农业产业技术体系专项(nycytx-009)

摘　　要：通过构建DNA池和测序,分析了广东蓝塘猪、大花白猪和外来长白猪3个猪种的乳铁蛋白基因启动子区和部分5′UTR区1 494 bp的单核苷酸多态性。结果表明,在3个猪种中发现了24处单核苷酸突变,其中12处为转换,8处为颠换,3处为插入,1处为缺失突变。通过生物信息学方法预测了猪乳铁蛋白基因启动子转录因子结合位点,发现了5个SNP:-1032A>G、-989C>T、-740T>G、-732G缺失、-680A>G,分别位于PEA3、cAMP、STAT5、SRY转录因子结合位点上,其中-1032 A>G产生了MYT1结合位点,-680A>G导致了SRY结合位点消失,引起了转录因子结合位点的改变,但是否对启动子转录活性有影响还需要进行深入研究。Based on the DNA pooling and sequencing, the single nucleotide polymorphism of the promoter and part 5＇ untranslated region of lactoferrin gene in 3 porcine breeds （Lantang, Dahuabai and Landraee） was studied. The results showed that in the sequence of 1 494 bp, 24 mutations were detected, of which 12 mutations were transitions, 8 were transversion, 3 were insertion, and 1 was deletion. The potential transcription factor binding sites were predicted and were analyzed with SNPs. And the results showed that there were many transcription factor binding sites in the promoter. Five SNPs, -1032A〉G,-989C〉T,-740T〉G, -732G（deletion） and -680A〉G were found in the PEAE3, cAMP, STATS and SRY sites, respectively. The SNPs caused the changes of the DNA sequences on the sites, but whether the SNPs could influence the gene expression need further research.

关 键 词：猪 乳铁蛋白基因 启动子 单核苷酸多态性 

分 类 号：S828[农业科学—畜牧学] Q754[农业科学—畜牧兽医]