板蓝根多糖对体外培养鸡脾脏淋巴细胞蛋白激酶C活性的影响  

Effect of Indigowoad Root Polysaccharides on The Activity of Protein Kinase C of Spleen Lymphocytes in Chicken

作　　者：刘俊玲[1] 张元元[1] 崔保安[1] 张红英[1] 王学兵[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《中国农学通报》2011年第11期15-19,共5页Chinese Agricultural Science Bulletin

基　　金：国家博士点基金项目"板蓝根多糖对畜禽免疫系统信号转导影响研究"(20070466001);郑州市创新型科技人才队伍建设工程资助项目(10CXTD148)

摘　　要：采用水提醇沉法提取板蓝根多糖,应用蒽酮-硫酸法进行多糖含量测定。体外培养鸡脾脏淋巴细胞,用固相夹心酶联免疫吸附试验(ELISA)测定蛋白激酶c(PKC)活性,分析板蓝根多糖对鸡脾脏淋巴细胞PKC活性的影响。结果显示,加入板蓝根多糖组(100mg/mL)与空白对照组的PKC的活性有显著差异(P<0.05),表明板蓝根多糖能引起鸡脾脏淋巴细胞蛋白激酶c活性明显升高。Indigowoad Root Polysaccharides（IRPS） was extracted by experimental boil alcohol extracti,using Anthrone-Sulfuric Acid Method for the Determination of Polysaccharides.Then cultivated Chicken Spleen lymphocytes in vitro to detect the Activity of Protein Kinase C（PKC） using Solid-Phase Sandwich Enzyme-Linked Immunosorbent Assay（ELISA） techniques and analyzed the effect of Indigowoad Root Polysaccharides on the Activity of Protein kinase C of Spleen lymphocytes in Chicken.The results showed that IRPS（100 mg/mL） can lead to Protein Kinase C of Splenic Lymphocytes was Significantly higher than the Control group which did not contain the Indigowoad Root Polysaccharides.

关 键 词：板蓝根多糖 鸡 脾脏淋巴细胞 蛋白激酶C 

分 类 号：S853.74[农业科学—临床兽医学]