沙漠中褐沙蒿根际细菌群落结构特征分析  

Characterization for Rhizobacterial Community Structure of Artemisia intramongolica in Deserts

作　　者：冯福应[1] 刘发来[1] 孙丽鹏[1] 孟建宇[1] 秦玉英 

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物实验所,呼和浩特010018 [2]内蒙古蒙牛乳业集团股份有限公司,呼和浩特011500

出　　处：《中国农学通报》2011年第11期271-275,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"内蒙古荒漠土壤纤维素降解菌分子生态学研究"(30860015);内蒙古自治区自然科学基金项目"木质纤维素低温高效降解微生物筛选及基因组改组"(2010BS0506)

摘　　要：基于16S rDNA基因的文库构建和分析,通过对比分析了浑善达克和毛乌素沙漠中褐沙蒿根际细菌群落结构特点。结果表明:褐沙蒿根际细菌类群主要有9类,即芽单胞菌(Gemmatimonadetes)(4.26%/2.08%,浑善达克样/毛乌素沙样,下同)、酸杆菌(Acidobacteria)(2.15%/2.08%)、放线菌(Actinobacteria)(10.64%/8.33%)、拟杆菌(Bacteroidetes)(2.13%/4.17%)、绿弯菌(Chloroflexi)(0/2.08%)和α-变形菌(α-proteobacteria)(65.96%/56.25%)、β-变形菌(β-proteobacteria)(0/6.25%)、δ-变形菌(δ-proteobacteria)(2.08%/2.13%)和γ-变形菌(γ-proteobacteria)(10.64%/2.08%)。来自2个采样地的褐沙蒿根际细菌菌群结构总体相似,表明植物在此结构组成决定方面起了重要作用;两地根际细菌菌群最主要差别之一是毛乌素褐沙蒿根际细菌比浑善达克沙漠多了3种光合细菌,即红螺菌(Rhodospirillales)、红细菌(Rhodobacter)和绿弯菌。Analysis and construction of library based on 16S rDNA gene,this study comparatively characterize the rhizobacterial community structure of Artemisia intramongolica sampled from Deserts of Hunsaderk and Mowosu.The results showed that rhizobacteria of Artemisia intramongolica composed of nine groups,i.e.Gemmatimonadetes（4.26%/2.08%,Hunsaderk/Mowos,the same with followings）,Acidobacteria（2.15%/2.08%）,Actinobacteria（10.64%/8.33%）,Bacteroidetes（2.13%/4.17%）,Chloroflexi（0/2.08%）,α-proteobacteria（65.96%/56.25%）,β-proteobacteria（0/6.25%）,δ-proteobacteria（2.08%/2.13%） and γ-proteobacteria（10.64%/2.08%）.Similar rhizobacterial community structure suggested that the associated plant was crucial for rhizobacteria composition,but some important difference between the plants from two deserts were observed,one of which was some photosynthetic bacteria existed in rhizosphere of Artemisia intramongolica from Mowosu while not in that from Hunsaderk.

关 键 词：褐沙蒿 根际细菌 菌群结构 16S RDNA 

分 类 号：S917.1[农业科学—水产科学]