猪生长激素ELISA检测体系的建立  

Establishment of ELISA Testing System of Porcine Growth Hormone (pGH)

作　　者：孔金超[1,2] 宋海龙[1] 方南洙[2] 兰海楠[1] 郑鑫[1] 

机构地区：[1]延边大学农学院,吉林龙井133400 [2]吉林农业大学动物科技学院,长春130118

出　　处：《中国农学通报》2011年第20期14-18,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"利用噬菌体表面展示技术淘选具有生物活性的猪生长激素模拟肽"(30871842)

摘　　要：为进行猪生长激素检测体系的探索研究,分别以自制猪生长激素单克隆抗体及兔抗猪生长激素IgG就影响检测ELISA(酶联免疫吸附法)体系因素进行探讨。结果显示,猪生长激素单克隆抗体的最适操作参考值为:最佳单抗包被的浓度为3μg/mL,4℃过夜为最佳包被条件,羊血清采用5%浓度封闭1h,最佳兔抗猪生长激素IgG的工作浓度为2μg/mL。Exploration and research for the testing system of porcine growth hormone, the author to make a pGH and rabbit monoclonal antibody IgG anti-porcine growth hormone to objective to analyze the influential factors of pGH by ELISA （enzyme-linked immunosorbent assay） testing system. The results showed that the optimum operation reference value of porcine growth hormone monoclonal antibodies was： the optimum coating antibody concentration was 3 μg/mL, coated overnight at 4℃ condition, 5% goat serum closed 1 h, the best work concentration of rabbit IgG anti-porcine growth hormone was 2 μg/mL.

关 键 词：猪生长激素 酶联免疫吸附法体系 单克隆抗体 

分 类 号：S852.4[农业科学—基础兽医学]