蟾酥注射液佐剂对鸡新城疫、传染性支气管炎二联活疫苗免疫效果的影响  

The Influence of Venenum Bufonis Injection Adjuvant Immune Effect on Newcastale Disease and Infectious Bronchitis Live Vaccine

作　　者：李敬双[1] 于洋[1] 唐雨顺[1] 张玉科[1] 李明[1] 田莉莉[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001

出　　处：《中国农学通报》2011年第20期71-75,共5页Chinese Agricultural Science Bulletin

基　　金：辽宁省教育厅科学技术研究项目"中药蟾酥抗鸡马立克氏病肿瘤机制的研究"(L2010336)

摘　　要：为了观察蟾酥注射液对鸡新城疫、传染性支气管炎二联活疫苗的调节作用,采用中药免疫药理学方法,选择不同浓度的蟾酥注射液与鸡新城疫、传染性支气管炎二联活疫苗联合使用,作为试验组。于7、14、21、28、35、42、49、56日龄,通过静脉采集血液,采用MTT法,检测外周血T、B淋巴细胞吸光度值(OD值);采用β-微量法,检测血清中新城疫和传染性支气管炎抗体效价。结果显示,所有试验组中,蟾酥注射液均能够显著提高外周血T、B淋巴细胞OD值,中剂量组(0.25mg/mL)T、B淋巴细胞OD值最大;蟾酥注射液能够显著提高血清中新城疫和传染性支气管炎抗体效价,中剂量组免疫后期抗体存在时间长,抗体高峰期持续时间也长。因此,说明蟾酥注射液是鸡新城疫、传染性支气管炎二联活疫苗有效的免疫佐剂。In the present study, researchers observed the regulating action of venenum bufonis injection on Newcastale disease （ND） and infectious bronchitis （IB） live vaccine. Methods： researchers choose the traditional Chinese immunopharmacology method and combined different concentration venenum bufonis injection and ND and IB live vaccine as test groups. On the 7th, 14th, 21th, 28th, 35th, 42th, 49th and 56th day after immunization, researchers took blood samples through vein and choose the MTT method to test the optical density（ OD ） of peripheral blood T cells and B cells. Researchers took β -micromethod to test the antibodytiter of ND and IB in blood serum. The results showed that in the all of the test groups, venenum bufonis injection could observably increase OD of peripheral blood T and B cells. In the medium dosage group （0.25 mg/mL）, the OD of T cells and B cells was the highest. Venenum bufonis injection could significantly increase the antibodytiter of ND and IB in blood serum. In the medium dosage group （0.25 mg/mL）, antibody existence lasted longer in late immunization and antibody peak hour lasted longer too. Therefore, venenum bufonis injection was the effective adjuvant of ND and IB live vaccine.

关 键 词：蟾酥注射液 佐剂 疫苗 免疫效果 

分 类 号：S859[农业科学—临床兽医学]