鸡源性大肠杆菌产超广谱β-内酰胺酶基因型分布  

Genotype Distribution of Extended-Spectrum β-Lactamases Produced by Escherichia coli Isolated from the Fowl in Dalian Area of Liaoning

作　　者：张利勃[1] 刘延庆 乔长涛[1] 高慎阳[1] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001 [2]黑龙江省讷河市畜牧水产局,黑龙江讷河161300

出　　处：《中国农学通报》2011年第20期76-78,共3页Chinese Agricultural Science Bulletin

基　　金：辽宁省教育厅高等学校科学研究项目"PRRSV辽宁分离株遗传变异分析及分子诊断方法研究"(L2010265)

摘　　要：检测鸡大肠杆菌超广谱β-内酰胺酶(ESBLs)的基因型并了解辽宁省鸡源性大肠杆菌产ESBLs基因型分布。对大连4个地区不同鸡场临床分离32株产ESBLs鸡大肠杆菌分别用TEM、OXA、CTX-M和SHV等4种通用引物进行PCR扩增确定其基因型。结果显示,32株鸡大肠杆菌均检出TEM型基因、12株检出CTX-M型基因、2株检出OXA型、未检出SHV型;TEM+CTX-M双阳性检出11株、TEM+OXA双阳性检出2株。TEM基因亚型占检出比例最高(100%)。目前辽宁省产ESBLs鸡大肠杆菌基因亚型以TEM为主,其次为CTX-M型,未发现SHV型;以TEM+CTX-M双阳性检出率最高(34.4%)。To elucidate the genotype distributions of ESBLs produced by E. coli isolated from the fowl in Liaoning Province. Thirty-two ESBL-producing Escherichia coli were collected from four different chicken farms in Dalian, and were analyzed by PCR amplifying using the primers of TEM, OXA, SHV and CTX-M, respectively. The results showed that： thirty-two strains with TEM-type, twelve CTX-M-types and two OXA-types were detected except SHV-type was not found; moreover, eleven strains with multiple TEM ＋ CTX-M and two strains with TEM＋OXA genotypes were detected. The predominant genotype of ESBLs was TEM （100% detection rate） in chicken farms of Liaoning Province, and then were CTX-M, SHV was not found; the multiple TEM＋CTX-M positive rate reached up to 34.4%.

关 键 词：鸡大肠杆菌 超广谱Β-内酰胺酶 TEM型基因 CTX-M型基因 

分 类 号：S855.1[农业科学—临床兽医学]