余甘子多糖提取物抗氧化活性研究  

The Study on the Antioxidant Activity of Polysaccharide Extract from Phyllanthus emblica L.

作　　者：高路[1,2] 张公信[3] 高云涛[2,3] 

机构地区：[1]云南民族大学科技处,昆明650500 [2]民族药资源化学国家民委-教育部重点实验室,昆明650500 [3]云南民族大学化学与生物技术学院,昆明650031

出　　处：《中国农学通报》2011年第20期133-136,共4页Chinese Agricultural Science Bulletin

基　　金：云南省社会发展科技计划项目"醇-盐双水相体系制备植物多酚及抗氧化活性研究"(2007B148M);民族药资源化学国家民族事务委员会-教育部共建重点实验室开放基金"傣族雅解药竹叶兰类黄酮分离分析及活性研究"(MJY090101);云南省教育厅科学研究基金项目"傣族雅解竹叶兰抗氧化活性成分研究"(2010C213)

摘　　要：利用超声提取-水提醇沉法从余甘子中提取可溶性多糖,多糖提取率达4.61%。提取物中多糖含量为44.62%。考查了余甘子多糖提取物的抗氧化活性,对Fenton反应羟自由基清除作用的IC50为0.38mg/mL,最大清除率为80.3%,对光照核黄素超氧阴离子自由基清除作用的IC50为0.93mg/mL,最大清除率为85.9%,对羟自由基诱发卵磷脂脂质过氧化损伤的IC50为0.39mg/mL,最大抑制率为71.3%。实验结果表明余甘子富含活性多糖,余甘子多糖具有良好的抗氧化作用,最大清除作用与茶多酚相近,具有较好的研究价值。The water soluble polysaccharide was extracted from phyllanthus emblica L. by the method of ultrasound-water extracting-alcohol precipitating. Polysaccharide extraction yield of 4.61% was obtained and the content of polysaccharide in the extract was reached to 44.62% . The antioxidant activity of the polysaccharide of phyllanthus emblica L. was investigated. The IC 50 of scavenging effect on hydroxyl free radical generated by Fenton reaction was 0.38 mg/mL, the maximum scavenging rate was 80.3%. The IC 50 of scavenging effect on superoxide radical generated by riboflavine photosensitization was 0.93 mg/mL, the maximum scavenging rate was 85.9%. The IC 50 of inhibition effect on lecithin lipid peroxidation induced by hydroxyl free radical was 0.39 mg/mL, the maximum inhibition rate was 71.3% . The results showed that phyllanthus emblica L. polysaccharides had good antioxidant activity, which was closed to tea polyphenols.

关 键 词：余甘子 多糖 抗氧化 

分 类 号：R285.5[医药卫生—中药学]