高效液相色谱法测定桂花中毛蕊花苷的含量  

Determination of verbascoside in Osmanthus fragrans by HPLC

作　　者：丁立新[1] 王心合[1] 杜永祥[1] 柴佳丽[1] 

机构地区：[1]佳木斯大学药学院(黑龙江省生物药制剂重点实验室),黑龙江佳木斯154007

出　　处：《中国当代医药》2011年第21期9-10,共2页China Modern Medicine

摘　　要：目的:建立HPLC法测定桂花中毛蕊花苷含量的方法。方法:采用ODSC18柱(250mm×4.6mm,5μm),以乙腈-0.2%冰醋酸溶液(10∶35)为流动相,流速1ml/min,检测波长为310nm。结果:该方法线性关系良好,毛蕊花苷平均回收率为99.83%,RSD为0.40%。结论:该方法快速、简便、准确、重现性好,可用于桂花中毛蕊花苷的质量控制。Objective： To establish a method for determining the verbascoside in Osmanthus fragrans by HPLC. Methods： ODSC18 column （250 mm×4.6 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-0.2% phosphoric acid（1∶3.5）. The flow rate was 1.0 ml/min. The detection wavelength was set at 310 nm. Rseults： The linear relation was good. The average recovery of verbascoside was 99.83% with RSD=0.40%. Conclusion： The method is rapid, simple, accurate and has good reproducibility. It can be used for the quality control of the verbascoside in Osmanthus fragrans.

关 键 词：桂花 毛蕊花苷 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]