抗氧化剂NAC抑制新生大鼠脑缺氧缺血损伤相关的神经细胞凋亡  

ANTIOXIDANT INHIBITS NEURONAL CELL APOPTOSIS ASSOCIATED WITH CEREBRAL HYPOXIA-ISCHEMIA IN THE NEONATAL RAT

作　　者：黄晓明[1] 胡向阳[1] 蒋犁[1] 

机构地区：[1]南京铁道医学院科研所分子生物学研究室,210009

出　　处：《新生儿科杂志》1999年第6期260-261,267,共3页The Journal of Neonatology

摘　　要：目的　研究抗氧化剂 (NAC)对缺氧缺血脑损伤引起的神经细胞凋亡的作用。方法　利用新生大鼠缺氧缺血脑损伤的动物模型 ,通过末端转移酶介导的原位缺口标记 (TUNEL)法 ,检测并比较在有或无药物保护下脑皮质、海马中神经细胞凋亡的程度。结果　抗氧化剂(NAC)处理显著减少缺氧缺血脑组织中凋亡阳性细胞数。结论　抗氧化剂 NAC可有效抑制缺氧缺血脑损伤引起的神经细胞凋亡 ;Institute of Medical Sciences, Nanjing Railway Medical College,Nanjing,210009.\;Objevtive To examine the effect of antioxidant (N acetyl L Cysteine, NAC) in neuronal apoptosis associated with cerebral hypoxic ischemic injury.Methods Terminal deoxynucleotidyl transferase mediated dUTP nick end labdciy (TUNEL) was used to detect DNA cleavage due to apoptosis in brain paraffin section from bypoxic ischemic neonatal rats, and the stained nuclei in cerebral cortex and hippocampus were observed and counted. Results Brain samples of hypoxic ischemic neonatal rats pretreated with antioxidant exhibited significantly decreased numbers of positive statined neclei compared to untreated brain tissue. Conclusion Antioxidant (NAC) inhibits effectively neuronal cell apoptosis associated with cerebral hypoxia ischemia in the neonatal rat; Oxidative stress is implicated in the mechanism of neonatal hypoxic schemic neuronal damage. (Original article on page 260)

关 键 词：抗氧化剂 NAC 细胞凋亡 新生儿 缺氧缺血性脑病 

分 类 号：R722.120.5[医药卫生—儿科]