荧光探针可视化检测水溶液中铜离子  

A Sensitive Probe for Determination of Cu^(2+) With Optical Detection in Aqueous Solution

作　　者：胡明明[1] 樊江莉[1] 李宏林[1] 帕哈丁[1] 泽耀东[1] 彭孝军[1] 

机构地区：[1]大连理工大学精细化工国家重点实验室,大连116024

出　　处：《分析化学》2011年第8期1195-1200,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.20725621;21076032;20923006);国家重点基础研究发展计划(No.2009CB724706);教育部科技创新工程重大项目培育资金(No.707016);中央高校基本科研业务费专项资金(DUT10LK05)资助项目

摘　　要：设计并合成了一例罗丹明B衍生物D2。作为专一识别Cu2+的探针,在2×10-5 mol/L HEPES-CH3CN(1∶1,V/V,含0.1mol/L NaNO3,pH=7.4)溶液中,D2本身无颜色,荧光微弱;加入Cu2+后,溶液很快呈现粉红色,并且荧光增强2.7倍,而对其它常见金属离子都没有响应。紫外吸收光谱显示D2对较低浓度Cu2+的检测仍呈良好的线性关系,检出限为1.29×10-7 mol/L。对于1×10-6 mol/L Cu2+仍可以实现裸眼可见的颜色变化,制成试纸可检测mg/L级别含Cu2+水样。本方法对于实际环境中Cu2+的快速实时检测具有潜在的应用价值。A novel Rodamine B derivative D2 was designed and synthesized as a specific probe for Cu2＋.D2 itself has no color and weak fluorescence in 2×10-5 mol/L 4-（2-hydroxyerhyl）piperazine-1-erhanesulfonic acid（HEPES）-CH3CN（1 ∶ 1,V/V,0.1 mol/L NaNO3,pH=7.4） solution;after added Cu2＋,the solution turned into pink color quickly,as well as 2.7-fold fluorescence enhancement,while other common metal ions were not responding.The UV detection limit was 1.29×10-7 mol/L.The color change reduced by 1×10-6 mol/L Cu2＋ still can be seen by naked eyes.D2 can be loaded as test paper for detecting Cu2＋ with mg/L level in water solution.This method has potential application for detection of Cu2＋ in the actual environment with rapid real-time analysis.

关 键 词：罗丹明B 铜离子 可逆性 探针 

分 类 号：TP274[自动化与计算机技术—检测技术与自动化装置]