基于冠心病心肌缺血血瘀证小型猪血清核磁共振代谢组学的研究  

Study of Mini-pig Serum of Coronary Heart Disease(Chronic Myocardial Ischemia) with Syndrome of Blood Stasis Based on Nuclear Magnetic Resonance Metabolomics

作　　者：王勇[1] 李中峰[2] 陈建新[1] 啜文静[1] 李春[1] 郭淑贞[1] 赵慧辉[1] 王伟[1] 王英锋[2] 

机构地区：[1]北京中医药大学药理教研室,北京100029 [2]首都师范大学化学系,北京100037

出　　处：《分析化学》2011年第8期1274-1278,共5页Chinese Journal of Analytical Chemistry

基　　金：国家科技重大专项(No.2009ZX09502-018);国家自然科学基金(No.30902020)资助

摘　　要：通过左冠状动脉前降支放置Ameriod环制备冠心病慢性心肌缺血动物模型,并结合冠脉造影等方法确立中医血瘀证证候,采用现代核磁共振(NMR)和模式识别技术,分析冠心病心肌缺血血瘀证模型与假手术组小型猪4星期血清的共振氢谱,及相应代谢物谱群的变化,开展中医血瘀证候学的研究。结果显示,该方法制备的模型4星期后确立为稳定的冠心病慢性心肌缺血血瘀证模型,PCA方法清晰地将模型组与假手术组动物分别,血清1 HNMR谱的主成分分析(PCA)、两组动物代谢谱各不相同。假手术组与模型组比较发现,血清中内源性代谢物柠檬酸、肌酸、γ-氨基丁酸、乳酸、肉碱、葡萄糖等含量上升,低密度脂蛋白(LDL)与极低密度脂蛋白(VLDL)等脂质代谢物的浓度下降。可见,在血瘀证早期糖代谢与脂类代谢紊乱,两者互为因果,导致能量代谢中三羧酸循环(TCA)平衡的破坏,最终加重并恶化了冠心病血瘀证的进程,这些代谢物的特征组合模式可成为临床诊断与防治冠心病血瘀证的诊疗指标。To study coronary heart disease（chronic myocardial ischemia） with syndrome of blood stasis caused by Ameriod ring and its changes in the serum metabolite profiling,coronary angiography is used to evaluate the syndrome of the model,and nuclear magnetic resonance（NMR） and pattern recognition to analyze hydrogen resonance spectra of myocardial ischemia in coronary heart disease with syndrome of blood stasis after operated for 4 weeks.The results show that the principal component analysis（PCA） of serum NMR spectrum and metabolic spectrum in two groups are significantly different.Compared with the sham group,endogenous metabolites in the serum in model group have these changes： the levels of citric acid,creatinine,γ-aminobutyric acid,lactic acid,carnitine and glucose rise,and the concentration of lipid metabolites such as LDL and VLDL decline.Glucose metabolism and lipid metabolism disorders reinforce each other,which results a deterioration of coronary artery disease.These metabolites pattern may be used as clinical diagnosis and treatment of coronary artery disease indicators.

关 键 词：冠心病 血瘀证 核磁共振 代谢组学 

分 类 号：R541.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]