高压氧对CO中毒大鼠海马中钙离子、cGMP及血浆NO含量的影响  

Effects of hyperbaric oxygen on the contents of [Ca 2+ ]i and cGMP in hippocampus and nitric oxide in plasma after carbon monoxide poisoning in rats

作　　者：高春锦[1] 赵立明[1] 夏成清[1] 武连华[1] 饶小雪[1] 庞宝森[1] 葛环[1] 

机构地区：[1]首都医科大学附属北京红十字朝阳医院,北京市100020

出　　处：《中华航海医学杂志》1999年第4期226-229,共4页

摘　　要：目的　测定一氧化碳（ＣＯ）中毒大鼠海马中钙离子（［Ｃａ２＋ ］ｉ）浓度与环磷酸鸟苷（ｃＧＭＰ）含量以及血浆一氧化氮（ＮＯ）含量的变化，同时观察高压氧（ＨＢＯ）在其间的作用。方法　８６ 只实验大鼠被随机分成１１ 组，分别为正常（对照）组；ＣＯ 中毒后１、３、５、１０、２０ 天５个组（ＣＯ组）；ＣＯ中毒后＋ ＨＢＯ１、３、５、１０、２０天５ 个组（ＣＯ＋ ＨＢＯ组）。分别用流式细胞仪测定大鼠海马中钙离子浓度和用酶免法测定海马神经元内ｃＧＭＰ含量，再用分光光度法测定大鼠血浆ＮＯ含量。结果　ＣＯ组海马中［Ｃａ２＋ ］ｉ浓度在ＣＯ中毒后第３、５、１０ 天和ｃＧＭＰ含量在第１、３、５、１０ 天明显低于对照组（Ｐ＜ ０．０１），均于第２０ 天接近对照组（Ｐ＞ ０．０５）；血浆ＮＯ浓度在中毒后第１、３、５、１０ 天明显低于对照组（Ｐ＜ ０．０１），至第２０ 天仍未恢复正常（Ｐ＜ ０．０５）。而ＣＯ＋ ＨＢＯ组海马中［Ｃａ２＋ ］ｉ浓度和ｃＧＭＰ含量以及血浆ＮＯ浓度在ＣＯ中毒后虽有变化但与ＣＯ组比，各时期数值均较接近对照组。并在第１０ 天后恢复正常。结论　ＣＯ中毒后，大鼠海马神经元细胞内钙离子、ｃＧＭＰ与血浆ＮＯ含量均降低，并持续较长时间。Objective To observe the effects of hyperbaric oxygen(HBO)on the changes of [Ca 2+ ]i and cGMP contents in hippocampus and nitric oxide(NO) level in plasma after carbon monoxide(CO) poisoning in rats.Methods 86 rats were randomly divided into 11 groups,namely:5 CO groups,5 CO+HBO groups and 1 control group.All the rats of CO and CO+HBO groups were exposed to 3378.32 mg/L CO for 1 h,then the rats of CO+HBO groups were given HBO treatment after the CO exposure.[Ca 2+ ]i and cGMP in hippocampus were observed by means of flow cytometer and RIA at 1,3,5,10 and 20 d after the begining of the experiment;and NO in plasma was measured by spectrophotometer at the same time.Results The levels of [Ca 2+ ]i in CO groups at 3,5 and 10 d and cGMP at 1,3,5 and 10 d in hippocampus were significantly lower than those of the control group( P <0 01),then they approached the control level at 20 d( P >0 05).The Levels of NO in the plasma at 1,3,5 and 10 d were lower than the control and they still did not recover at 20 d( P <0 05).Although the levels of [Ca 2+ ]i and cGMP in hippocampus and NO in plasma in CO+HBO groups were also lower than the control,but they all approached control level at 10 d and after( P >0 05).Conclusions The levels of [Ca 2+ ]i,cGMP in hippocampus and NO in plasma are all decreased after CO poisoning.It is considered that NO change is the cause of the injury after CO poisoning and HBO has a positive effect on CO poisoning.

关 键 词：高压氧 一氧化碳中毒 钙 一氧化氮 治疗 

分 类 号：R959.105[医药卫生—药学] R459.6