Construction and Genetic Transformation of Over-Expression Vector of nad1 Gene in Rice  

水稻线粒体nad1基因超表达载体的构建和遗传转化(英文)

作　　者：李炜[1] 谭艳平[1] 王春台[1] 刘学群[1] 

机构地区：[1]中南民族大学生命科学院/生物技术国家民委重点实验室,武汉430074

出　　处：《Agricultural Science & Technology》2011年第7期963-965,共3页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(30871318)~~

摘　　要：[Objective] The aim was to clone the mitochondrial-related gene nad1 and produce transgenic rice plants with nad1.[Method] The total RNA was extracted from rice seedlings and reverse transcripted into cDNA.Then the target gene nad1 was amplified by using the cDNA as template.The nad1 and Rf1b,a sequence of signal peptide of mitochondria,were linked to binary expression vector pCAMBIA1305.1.The recombinant plasmid was transformed into the callus by Agrobacterium-mediated approach.[Result] The target gene nad1 was 978 bp.The binary expression vector carrying nad1 and signal peptide of mitochondria was constructed successfully.In addition,a lot of transgenic plants were obtained.[Conclusion] The study will provide basis to investigate the effect of over-expression of nad1 on rice plant growth.[目的]克隆线粒体相关基因nad1,获得转nad1的转基因水稻植株。[方法]采用TRIzol法提取水稻幼苗总RNA,以反转录的cDNA为模板,扩增得到nad1;将nad1接到线粒体信号肽Rf1b的5 (Rf1b5 ),装载到pCAMBIA1305.1双元表达载体,采用农杆菌介导的愈伤组织侵染法进行遗传转化。[结果]克隆的目的基因nad1大小为978 bp,成功构建了携带线粒体信号肽的nad1植物表达载体,并获得了转nad1基因的阳性植株。[结论]为探讨水稻中过表达nad1对水稻生长的影响奠定了基础。

关 键 词：RICE nad1 Genetic transformation 

分 类 号：S511[农业科学—作物学]