芹灵冲剂对DHBV肝炎雏鸭肝、肾功和血液生化指标的影响  

Effects of Qinling chongji on hepato renal function and blood biochemistry in DHBV hepatitis nestling ducks

作　　者：黄正明[1] 杨新波[1] 曹文斌[1] 陈鸿雁[1] 郑鸣[1] 张敬珍[1] 梁晓莉[1] 卢立军[1] 陈鸿珊[2] 李壮 汪得青 

机构地区：[1]中国人民解放军北京医学高等专科学校药理教研室病理教研室,北京市100071 [2]中国医学科学院医药生物技术研究所病毒室,北京市100085 [3]中国人民解放军301总医院生化检验科,北京市100830

出　　处：《世界华人消化杂志》1999年第12期1042-1043,共2页World Chinese Journal of Digestology

摘　　要：目的　观察芹灵冲剂（Ｑｉｎｌｉｎｇ ｃｈｏｎｇｊｉ， ＱＬ） 对ＤＨＢＶ 肝炎雏鸭肝脏的保护作用及对肝炎雏鸭肾功能、血液生化改变的影响．方法　待ＤＨＢＶ 感染雏鸭７ ｄ 血清检测为阳性后，随机分组进行药物治疗试验，每组５ ～６ 只，ＱＬ 分为高（ＱＬｂ） 、中（ＱＬｍ） 、低（ＱＬｓ）３ 个剂量组，分别为１０ ，５ 和２－５ ｇ／ ｋｇ 组，ｉｇ ，２ 次／ ｄ ，１０ ｄ ． 设病毒对照组（ＤＨＢＶ） ，以生理盐水代替药物． 阳性药用阿昔洛韦（ 无环鸟苷，ＡＣＶ） 粉剂１００ ｍｇ／ ｋｇ ，ｉｇ ．２ 次／ ｄ，１０ ｄ ，自鸭腿胫静脉取血，分离血清，用全自动生化测定仪分别测定肝功能（ＡＬＴ，ＡＳＴ，ＳＢ） 、肾功能（ ＵＮ，ＣＲ） 及其他生化指标的含量． 并将各组动物采血后剖腹取出肝脏，在肝脏同一部位取样，１００ ｍ Ｌ／ Ｌ中性福尔马林液固定，常规石蜡包埋切片，ＨＥ 染色，光镜观察．结果　雏鸭被ＤＨＢＶ 感染７ ｄ 后血液生化指标明显异常，其中肝功能（ＡＬＴ，ＡＳＴ，ＳＢ） 和肾功能（ＵＮ，ＣＲ） 改变最明显，其次乳酸脱氢酶（ＬＤＨ） 显著升高． 用药组经不同浓度的ＱＬ 分别治疗１０ ｄ 后，尤其ＱＬｂ（１０ ｇ／ ｋｇ） 对上述异常升高的血液生化指标均显?AIM To observe the protective effect of Qinling chongji (QL) on liver and renal functions, and the effect on blood biochemistry in DHBV induced hepatitis nestling ducks. METHODS When positive serum DHBV was detected in seven day infected nestling ducks, animals were randomly divided into five groups: ①normal saline (NS); ②positive control drug, ACV 100*!mg·kg -1 . ③ QL 10*!g·kg -1 (QLb); ④QL 5g·kg -1 (QLm);⑤ QL 2 5*!g·kg -1 (QLs). Five to six animals were used in each group. Drugs were administered orally, twice daily for 10 days. The blood samples were taken from leg vein 10 days after treatment and sera were separated at once. RESULTS Blood biochemical indexes of nestling ducks showed significant abnormality 7 days after DHBV infection, of which liver function (ALT, AST, SB)and renal function (UN, CR) changed the most, secondly,LDH level increased significantly. The animals were treated with different doses of QL for 10 days, above normal biochemical indexes were lowered in QLb (10*!g·kg -1 ) group ( P <0 05 , P <0 01 ). Liver pathological examination demonstrated that the constructions of liver leaflet in DHBV control group were damaged completely, while those in QLb group maintained intact. CONCLUSION QL can protect liver tissues in DHBV induced hepatitis nestling duck and could decrease the serum levels of ALT, AST, SB, UN and CR, especially the later two. The liver and renal functions were improved after treatment with QL, so it is worthwhile to perform further clinical studies on this anti hepatitis drug.

关 键 词：芹灵冲剂 乙型肝炎病毒 病理学 中医药疗法 

分 类 号：R259.126.2[医药卫生—中西医结合]